A genomic analysis and transcriptomic atlas of gene expression in Psoroptes ovis reveals feeding- and stage-specific patterns of allergen expression
| Autor: | Stephane Rombauts, Stewart T. G. Burgess, Jackie Dunn, Thomas Van Leeuwen, Yves Van de Peer, Robert J. Weaver, Alasdair J. Nisbet, Rachel E. Down, Jessica C. Prickett, Kathryn Bartley, Edward J. Marr, Francesca Nunn |
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| Rok vydání: | 2019 |
| Předmět: |
Male
Psoroptes ovis ACARI Proteomics Genome 0302 clinical medicine Gene expression Cluster Analysis Ovis Phylogeny DERMATOPHAGOIDES-FARINAE Genetics 0303 health sciences biology Sheep scab Gene Expression Regulation Developmental Genomics FAMILY WEB ARGININE KINASE Multigene Family Female DNA microarray Research Article Biotechnology lcsh:QH426-470 PROTEINS lcsh:Biotechnology 030231 tropical medicine Development 03 medical and health sciences Sex Factors SERPIN lcsh:TP248.13-248.65 Animals Gene 030304 developmental biology Life Cycle Stages Sheep Life-cycle IDENTIFICATION Gene Expression Profiling Psoroptidae Biology and Life Sciences SHEEP SCAB MITE Feeding Behavior Allergens biology.organism_classification EVOLUTION lcsh:Genetics Transcriptome |
| Zdroj: | BMC GENOMICS BMC Genomics BMC Genomics, Vol 20, Iss 1, Pp 1-23 (2019) |
| ISSN: | 1471-2164 |
| Popis: | BackgroundPsoroptic mange, caused by infestation with the ectoparasitic mite,Psoroptes ovis, is highly contagious, resulting in intense pruritus and represents a major welfare and economic concern for the livestock industry Worldwide. Control relies on injectable endectocides and organophosphate dips, but concerns over residues, environmental contamination, and the development of resistance threaten the sustainability of this approach, highlighting interest in alternative control methods. However, development of vaccines and identification of chemotherapeutic targets is hampered by the lack ofP. ovistranscriptomic and genomic resources.ResultsBuilding on the recent publication of theP. ovisdraft genome, here we present a genomic analysis and transcriptomic atlas of gene expression inP. ovisrevealing feeding- and stage-specific patterns of gene expression, including novel multigene families and allergens. Network-based clustering revealed 14 gene clusters demonstrating either single- or multi-stage specific gene expression patterns, with 3075 female-specific, 890 male-specific and 112, 217 and 526 transcripts showing larval, protonymph and tritonymph specific-expression, respectively. Detailed analysis ofP. ovisallergens revealed stage-specific patterns of allergen gene expression, many of which were also enriched in “fed” mites and tritonymphs, highlighting an important feeding-related allergenicity in this developmental stage. Pair-wise analysis of differential expression between life-cycle stages identified patterns of sex-biased gene expression and also identified novelP. ovismultigene families including known allergens and novel genes with high levels of stage-specific expression.ConclusionsThe genomic and transcriptomic atlas described here represents a unique resource for the acarid-research community, whilst the OrcAE platform makes this freely available, facilitating further community-led curation of the draftP. ovisgenome. |
| Databáze: | OpenAIRE |
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