A New Artificial Antigen of the Hepatitis E Virus
| Autor: | Yu. E. Khudyakov, A. N. Burkov, A. P. Obriadina, T. I. Ulanova, Howard A. Fields, G. Talekar |
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| Rok vydání: | 2008 |
| Předmět: |
Artificial antigen
viruses Molecular Sequence Data Clinical Biochemistry Immunology Mosaic protein Oligonucleotides Biology Protein Engineering medicine.disease_cause Mice Viral Proteins Hepatitis E virus Antigen medicine Animals Humans Immunology and Allergy Amino Acid Sequence Hepatitis Antibodies Gene Base Sequence Oligonucleotide Hepatitis Antigens virus diseases Serum samples Hepatitis E medicine.disease Virology Molecular biology Recombinant Proteins digestive system diseases Medical Laboratory Technology |
| Zdroj: | Journal of Immunoassay and Immunochemistry. 30:18-39 |
| ISSN: | 1532-4230 1532-1819 |
| DOI: | 10.1080/15321810802570269 |
| Popis: | An artificial antigen composed of 12 small antigenic regions derived from the ORF2 and ORF3 HEV proteins was designed. The gene encoding for this artificial antigen was assembled from synthetic oligonucleotides by a new method called Restriction Enzyme-Assisted Ligation (REAL). The diagnostic relevance of this second generation HEV mosaic protein (HEV MA-II) was demonstrated by testing this antigen against a panel of 142 well defined anti-HEV positive and anti-HEV negative serum samples. The data obtained in this study support the substantial diagnostic potential of this HEV mosaic antigen. |
| Databáze: | OpenAIRE |
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