Affinity liquid chromatography and capillary electrophoresis of seminal plasma proteins
| Autor: | Marie Tichá, Tereza Varilova, Vera Pacakova, Hana Semenkova, Pavel Horák, Milan Madera, Karel Štulík |
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| Rok vydání: | 2006 |
| Předmět: |
Male
Chromatography Resolution (mass spectrometry) Heparin Elution Seminal Plasma Proteins Phosphorylcholine Chemistry Electrophoresis Capillary Filtration and Separation High-performance liquid chromatography Chromatography Affinity Analytical Chemistry Electrophoresis Capillary electrophoresis Biochemistry Affinity chromatography Animals Humans Protein Isoforms Chromatography Liquid |
| Zdroj: | Journal of Separation Science. 29:1110-1115 |
| ISSN: | 1615-9314 1615-9306 |
| DOI: | 10.1002/jssc.200500405 |
| Popis: | Interactions of boar, bull, and human seminal plasma proteins with heparin and phosphorylcholine were studied by affinity LC using heparin immobilized to a Toyopearl support. A step gradient elution from 0.15 to 1.50 M NaCl was employed to elute the seminal plasma proteins. Relative amounts of the heparin-binding fraction of seminal plasma proteins (H+) in seminal plasma of three species were determined. Further on, the fraction of seminal plasma proteins interacting with phosphorylcholine-binding proteins (P+) was evaluated. P+ proteins were not found in human seminal plasma and their highest amount was present in bull seminal plasma. A CE method was developed for separation of seminal plasma proteins. Various capillaries and separation conditions were tested; the best resolution was obtained in a bare-silica capillary, with a micellar system consisting of a 0.02 M borate buffer and 0.05 M SDS pH 10.0. The optimized conditions were applied to the identification of the components in boar plasma. |
| Databáze: | OpenAIRE |
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