Differential expression of miRNAs involved in biological processes responsible for inflammation and immune response in lichen sclerosus urethral stricture disease
| Autor: | Harjivan Kohli, Brandon Childs, Travis B. Sullivan, Artem Shevtsov, Eric Burks, Thomas Kalantzakos, Kimberly Rieger-Christ, Alex J. Vanni |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Science
Inflammatory Diseases Urology Immunology Plant Science Pathology and Laboratory Medicine Biochemistry Medical Conditions Signs and Symptoms Gastrointestinal Tumors Genetics Medicine and Health Sciences Non-coding RNA Immune Response Inflammation Natural antisense transcripts Multidisciplinary Biology and life sciences Prostate Cancer Prostate Diseases Cancers and Neoplasms Gene regulation Nucleic acids MicroRNAs Genitourinary Tract Tumors Gastric Cancer Oncology Lichenology Medicine RNA Gene expression Clinical Medicine Research Article |
| Zdroj: | PLoS ONE PLoS ONE, Vol 16, Iss 12, p e0261505 (2021) |
| ISSN: | 1932-6203 |
| Popis: | Purpose To better understand the pathophysiology of lichen sclerosus (LS) urethral stricture disease (USD), we aimed to investigate expression profiles of microRNAs (miRNAs) in tissue samples from men undergoing urethroplasty. Methods Urethral stricture tissue was collected from 2005–2020. Histologic features diagnostic of LS were the basis of pathologic evaluation. Foci of areas diagnostic for LS or non-LS strictures were chosen for RNA evaluation. In an initial screening analysis, 13 LS urethral strictures and 13 non-LS strictures were profiled via miRNA RT-qPCR arrays for 752 unique miRNA. A validation analysis of 23 additional samples (9 LS and 14 non-LS) was performed for 15 miRNAs. Statistical analyses were performed using SPSS v25. Gene Ontology (GO) analysis was performed using DIANA-mirPath v. 3.0. Results In the screening analysis 143 miRNAs were detected for all samples. 27 were differentially expressed between the groups (false discovery p-value 0.90 for distinguishing between between LS and non-LS strictures. 11-fold upregulation of MiR-155-5p specifically was found in LS vs. non-LS strictures (p Conclusions To our knowledge this is the first study evaluating miRNA expression profiles in LS and non-LS USD. We identified several miRNAs that are differentially expressed in USD caused by LS vs other etiologies, which could potentially serve as biomarkers of LS USD. The top eight differentially expressed miRNAs have been linked to immune response processes as well as involvement in wound healing, primarily angiogenesis and fibrosis. |
| Databáze: | OpenAIRE |
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