Separation of serum albumin by radial chromatography of nitrocellulose membranes
| Autor: | M. Kramlová, T.I. Pr̆istoupil |
|---|---|
| Rok vydání: | 1968 |
| Předmět: |
Serum albumin
Biochemistry Analytical Chemistry chemistry.chemical_compound Blood serum Methods Animals Humans Bovine serum albumin Cellulose Immunoelectrophoresis Serum Albumin Chromatography biology Organic Chemistry Membranes Artificial General Medicine Blood proteins Radial chromatography Membrane chemistry Blood chemistry biology.protein Serum Globulins Rabbits Nitrocellulose |
| Zdroj: | Journal of chromatography. 37(2) |
| Popis: | Radial chromatography of serum proteins was investigated under various experimental conditions on nitrocellulose membranes. Development of about 15 μg/0.5 μl samples on Sartorius 11002 filters at pH 8.0–9.0, and with a salt concentration of up to 5% NaCl at laboratory temperature (18–25°) was found most suitable for the small scale separation of serum albumin from the majority of other native or denatured serum proteins, within about 1 minute. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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