Heterochronic Protein Expression Patterns in the Developing Embryonic Chick Cerebellum
| Autor: | Lim Yh, Matthew K. Vickaryous, Gilbert Ea, Armstrong Cl |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Calbindins
Cerebellum Histology Proteome Purkinje cell Nerve Tissue Proteins Chick Embryo Biology Calbindin Mice Purkinje Cells S100 Calcium Binding Protein G Species Specificity medicine Animals Ecology Evolution Behavior and Systematics Gene Expression Regulation Developmental Anatomy Phenotype Embryonic stem cell Cell biology medicine.anatomical_structure Calbindin 2 embryonic structures Calretinin Heterochrony Neural development Biotechnology |
| Zdroj: | The Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology. 295:1669-1682 |
| ISSN: | 1932-8486 |
| DOI: | 10.1002/ar.22544 |
| Popis: | The advantages of the embryonic chick as a model for studying neural development range from the relatively low cost of fertilized eggs to the rapid rate of development. We investigated in ovo cerebellar development in the chick, which has a nearly identical embryonic period as the mouse (19–22 days). We focused on three antigens: Calbindin (CB), Zebrin II (ZII), and Calretinin (CR), and our results demonstrate asynchronous expression patterns during cerebellar development. Presumptive CB+ Purkinje cells are first observed at embryonic day (E)10 in clusters in posterior cerebellum. At E12, corresponding with global expression of CB across the cerebellum, Purkinje cells began to express ZII. By E14–E16, Purkinje cells disperse into a monolayer and develop a pattern of alternating immunopositive and immunonegative ZII stripes. CR is initially expressed by clusters of presumptive Purkinje cells in the nodular zone at E8. However, this expression is transient and at later stages, CR is largely confined to the granule and molecular layers. Before hatch (E18–E20), Purkinje cells adopt a morphologically mature phenotype with complex dendritic arborizations. Comparing this data to that seen in mice, we found that the sequence of Purkinje cell formation, protein expression, and development is similar in both species, but these events consistently begin ∼5–7 days earlier in the precocial chick cerebellum, suggesting an important role for heterochrony in neurodevelopment. Anat Rec, 2012. © 2012 Wiley Periodicals, Inc. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text