Sevoflurane induces cardioprotection through reactive oxygen species-mediated upregulation of autophagy in isolated guinea pig hearts

Autor: Toshiaki Minami, Genzou Takemura, Kazuhiro Kaneda, Mayumi Shiomi, Shizuka Koshinuma, Vincent M. Figueredo, Yoshihiro Momota, Anna Onishi, Yoshitaka Inamura, Masami Miyamae
Rok vydání: 2013
Předmět:
Zdroj: Journal of Anesthesia. 28:593-600
ISSN: 1438-8359
0913-8668
DOI: 10.1007/s00540-013-1755-9
Popis: Sevoflurane increases reactive oxygen species (ROS), which mediate cardioprotection against myocardial ischemia–reperfusion injury. Emerging evidence suggests that autophagy is involved in cardioprotection. We examined whether reactive oxygen species mediate sevoflurane preconditioning through autophagy. Isolated guinea pigs hearts were subjected to 30 min ischemia followed by 120 min reperfusion (control). Anesthetic preconditioning was elicited with 2 % sevoflurane for 10 min before ischemia (SEVO). The ROS-scavenger, N-(2-mercaptopropionyl) glycine (MPG, 1 mmol/l), was administered starting 30 min before ischemia to sevoflurane-treated (SEVO + MPG) or non-sevoflurane-treated (MPG) hearts. Infarct size was determined by triphenyltetrazolium chloride stain. Tissue samples were obtained after reperfusion to determine autophagy-related protein (microtubule-associated protein light chain I and II: LC3-I, -II) and 5′ AMP-activated protein kinase (AMPK) expression using Western blot analysis. Electron microscopy was used to detect autophagosomes. Infarct size was significantly reduced and there were more abundant autophagosomes in SEVO compared with control. Western blot analysis revealed that the ratio of LC3-II/I and phosphorylation of AMPK were significantly increased in SEVO. These effects were abolished by MPG. Sevoflurane induces cardioprotection through ROS-mediated upregulation of autophagy.
Databáze: OpenAIRE
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