DNA-directed in vitro synthesis of elongation factor Tu
| Autor: | Nathan Brot, David L. Miller, Tanya Schulz, Herbert Weissbach, Frederick Chu |
|---|---|
| Rok vydání: | 1976 |
| Předmět: |
DNA
Bacterial Transcription Genetic Biophysics Guanosine Tetraphosphate Peptide Elongation Factor Tu Biology Guanosine Diphosphate Biochemistry Antibodies chemistry.chemical_compound Transcription (biology) Immunoprecipitation Molecular Biology Incubation Antiserum Cell-Free System Templates Genetic Cell Biology Molecular biology In vitro Kinetics chemistry Protein Biosynthesis Chromatography Gel Electrophoresis Polyacrylamide Gel Peptides DNA EF-Tu Protein Binding |
| Zdroj: | Biochemical and Biophysical Research Communications. 73:917-927 |
| ISSN: | 0006-291X |
| Popis: | The in vitro synthesis of EF-Tu was studied using the transducing phage λrifd18 DNA as template. The EF-Tu synthesized was identified by its immunological properties, gel analysis, and its ability to interact with GDP and EF-Ts. Two other unidentified polypeptides were also precipitated from the incubation mixture by EF-Tu antiserum. The synthesis of total EF-Tu immunoprecipitable material was depressed about one-half by guanosine-5′-diphosphate-3′-diphosphate which was shown to inhibit the transcription process. |
| Databáze: | OpenAIRE |
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