Endodontic cements induce alterations in the cell cycle of in vitro cultured osteoblasts
| Autor: | S. Stea, Donatella Granchi, D. Cavedagna, Susanna Stea, Arturo Pizzoferrato, Gabriela Ciapetti |
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| Rok vydání: | 1995 |
| Předmět: |
Root canal
Dental Cements Dentistry Biology Cell Line Flow cytometry Calcium Hydroxide Root Canal Filling Materials chemistry.chemical_compound medicine Humans Propidium iodide Zinc Oxide-Eugenol Cement General Dentistry Analysis of Variance Osteoblasts medicine.diagnostic_test Cell growth business.industry Cell Cycle Osteoblast DNA Cell cycle Flow Cytometry Molecular biology In vitro medicine.anatomical_structure Otorhinolaryngology chemistry Cell culture Surgery Oral Surgery business Periapical Granuloma Cell Division |
| Zdroj: | Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology. 79:359-366 |
| ISSN: | 1079-2104 |
| DOI: | 10.1016/s1079-2104(05)80230-6 |
| Popis: | The effects of endodontic cements on the cell cycle of MG63 osteoblasts cultured in vitro have been examined. Three groups of compounds were tested. Group I encompassed zinc oxide- and eugenol-based cements (Tubliseal, Argoseal, N2), group II consisted of cements with a phenol group other than eugenol (AH26, Forfenan, Methode R/R), and group III included CaOH-based cements (Biocalex, Endocalex). The cell cycle of MG63 cells was analyzed by flow cytometry; the DNA content was evaluated by means of the propidium iodide uptake method, whereas the proportion of cells in the S phase was defined by the incorporation of bromodeoxyuridine later revealed by a specific antibody. The results showed that some root canal sealers could hamper the periapex healing processes by inhibiting the cell proliferation through a selective action on different phases of the cell cycle. |
| Databáze: | OpenAIRE |
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