Effects of grain, fructose, and histidine feeding on endotoxin and oxidative stress measures in dairy heifers
| Autor: | Ian J. Lean, A.R. Rabiee, Pietro Celi, H.M. Golder, R. King |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
medicine.medical_specialty
Rumen Fructose Poaceae medicine.disease_cause chemistry.chemical_compound Animal science Internal medicine Genetics medicine Animals Histidine Dry matter Dairy cattle chemistry.chemical_classification biology Glutathione peroxidase Body Weight Animal Feed Diet Endotoxins Oxidative Stress Endocrinology chemistry biology.protein Animal Nutritional Physiological Phenomena Cattle Female Animal Science and Zoology Edible Grain Ceruloplasmin Oxidative stress Food Science |
| Zdroj: | Journal of Dairy Science. 96:7881-7891 |
| ISSN: | 0022-0302 |
| DOI: | 10.3168/jds.2013-7113 |
| Popis: | Ruminal endotoxin and plasma oxidative stress biomarker concentrations were studied in dairy heifers challenged with grain, fructose, and histidine in a partial factorial study. Holstein-Friesian heifers [n=30; average body weight (BW) of 359.3±47.3 kg] were randomly allocated to 5 treatment groups: (1) control (no grain); (2) grain [crushed triticale at 1.2% of BW dry matter intake (DMI)]; (3) grain (0.8% of BW DMI) + fructose (0.4% of BW DMI); (4) grain (1.2% of BW DMI) + histidine (6g/head); and (5) grain (0.8% of BW DMI) + fructose (0.4% of BW DMI) + histidine (6 g/head). Rumen samples were collected by stomach tube 5, 65, 115, 165, and 215 min after diet consumption and blood samples at 5 and 215 min after consumption. Rumen fluid was analyzed for endotoxin concentrations. Plasma was analyzed for concentrations of the following oxidative stress biomarkers: reactive oxygen metabolites (dROM), biological antioxidant potential (BAP), advanced oxidation protein products, and ceruloplasmin, and activity of glutathione peroxidase. Dietary treatment had no effect on concentrations of endotoxin or oxidative stress biomarkers. We observed no interactions of treatment by time. Ruminal concentrations of endotoxin decreased during the sampling period from 1.12×10(5) ± 0.06 to 0.92×10(5) endotoxin units/mL ± 0.05 (5 and 215 min after diet consumption, respectively). Concentrations of dROM and the oxidative stress index (dROM/BAP × 100) increased over the sampling period, from 108.7 to 123.5 Carratelli units (Carr U), and from 4.1 to 4.8, respectively. Ceruloplasmin concentrations markedly declined 5 min after the consumption of diets, from 190 to 90 mg/L over the 215-min sampling period. Overall, a single feeding challenge for dairy cattle with grain, fructose, and histidine, and combinations thereof, may not be sufficient to induce marked changes in endotoxin or oxidative stress biomarker concentrations. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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