Snowflake-like DNA crystals templated Cu clusters as a fluorescent turn-on probe for sensing actin
| Autor: | Ting Liu, Huaiyu Bu, Hang Xu, Gang Xie, Xiangyuan Ouyang, Yongli Wu, Wang Meifang, Le Li, Ren Yong'an, Nan Jia |
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| Rok vydání: | 2021 |
| Předmět: |
Analytical chemistry
Metal Nanoparticles 02 engineering and technology 01 natural sciences Biochemistry Analytical Chemistry law.invention law Confocal microscopy Environmental Chemistry Spectroscopy Fluorescent Dyes Detection limit Chemistry 010401 analytical chemistry DNA 021001 nanoscience & nanotechnology Laser Fluorescence Actins 0104 chemical sciences Spectrometry Fluorescence Linear range 0210 nano-technology Selectivity Biosensor Order of magnitude Copper |
| Zdroj: | Analytica chimica acta. 1173 |
| ISSN: | 1873-4324 |
| Popis: | Herein, we synthesized snowflake-like DNA crystals (SDC) via hybridization chain reaction and used it for the first time in the synthesis of copper nanoclusters with enhanced fluorescence. Atomic force microscopy (AFM) and laser confocal microscopy characterization confirmed that SDC/CuNCs are self-assembled successfully on SDC. Aggregation induced emission allows SDC/CuNCs to exhibit better stability and stronger emission intensity. Thus, we developed the “turn-on” label-free fluorescence detection method of actin based on SDC/CuNCs which offer simplicity, low cost, good selectivity, and high sensitivity. The detection limit was determined to be 0.0124 μg mL−1, which was an order of magnitude lower than that of reported fluorescent methods (0.12 μg mL−1). Compared with previous method, the linear range is also much wider. We also performed standard recovery experiments in actual samples for evaluating the practicality of this strategy and proved that the capability of the proposed approach for the determination of actin is feasible and the interference from complex biological samples is negligible. These results indicate that SDC/CuNCs are expected to play a more important role in the field of biosensors. |
| Databáze: | OpenAIRE |
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