Contribution of runt-related transcription factor 2 to the pathogenesis of osteoarthritis in mice after induction of knee joint instability
| Autor: | Kozo Nakamura, Shingo Sato, Takashi Shimoaka, Toshihisa Komori, Satoru Kamekura, Ung-il Chung, Kazuto Hoshi, Hirotaka Chikuda, Yosuke Kawasaki, Shu Takeda, Hiroshi Kawaguchi, Zenjiro Maruyama, Gerard Karsenty |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
musculoskeletal diseases
Cartilage Articular Joint Instability Pathology medicine.medical_specialty Immunology Medial Collateral Ligament Knee Chondrocyte hypertrophy Core Binding Factor Alpha 1 Subunit Osteoarthritis Knee Joint Matrix metalloproteinase Menisci Tibial Pathogenesis Mice Rheumatology Matrix Metalloproteinase 13 medicine Immunology and Allergy Animals Pharmacology (medical) Collagenases RNA Messenger Mice Knockout Medial collateral ligament Chemistry musculoskeletal system medicine.disease Stifle Tibial Meniscus Injuries RUNX2 Mice Inbred C57BL Disease Models Animal medicine.anatomical_structure Medial meniscus Gene Deletion Collagen Type X |
| Zdroj: | Arthritis and rheumatism. 54(8) |
| ISSN: | 0004-3591 |
| Popis: | Objective By producing instability in mouse knee joints, we attempted to determine the involvement of runt-related transcription factor 2 (RUNX-2), which is required for chondrocyte hypertrophy, in the development of osteoarthritis (OA). Methods An experimental mouse OA model was created by surgical transection of the medial collateral ligament and resection of the medial meniscus of the knee joints of heterozygous RUNX-2–deficient (Runx2+/−) mice and wild-type littermates. Cartilage destruction and osteophyte formation in the medial tibial cartilage were compared by histologic and radiographic analyses. Localization of type X collagen and matrix metalloproteinase 13 (MMP-13) was examined by immunohistochemistry. Localization of RUNX-2 was determined by X-Gal staining in heterozygous RUNX-2–deficient mice with the lacZ gene insertion at the Runx2-deletion site (Runx2+/lacZ). Messenger RNA levels of type X collagen, MMP-13, and RUNX-2 were examined by real-time reverse transcriptase–polymerase chain reaction analysis. Results RUNX-2 was induced in the articular cartilage of wild-type mice at the early stage of OA, almost simultaneously with type X collagen but earlier than MMP-13. Runx2+/− and Runx2+/lacZ mice showed normal skeletal development and articular cartilage; however, after induction of knee joint instability, they exhibited decreased cartilage destruction and osteophyte formation, along with reduced type X collagen and MMP-13 expression, as compared with wild-type mice. Conclusion RUNX-2 contributes to the pathogenesis of OA through chondrocyte hypertrophy and matrix breakdown after the induction of joint instability. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text