Characterization and antibacterial mechanism of poly(aminoethyl) modified chitin synthesized via a facile one-step pathway
| Autor: | Dongsu Cha, Yan Wang, Guozhu Chang, Bing Fan, Chengsheng Liu, Wenjing Zhu, Shengnan Liang, Qifeng Dang, Hantian Sun, Yubei Zhang |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Polymers and Plastics
Chitin 02 engineering and technology 010402 general chemistry 01 natural sciences chemistry.chemical_compound Staphylococcus epidermidis Materials Chemistry Ethylamines Solubility Antibacterial agent Thermostability biology Organic Chemistry Cell Membrane 021001 nanoscience & nanotechnology biology.organism_classification 0104 chemical sciences Anti-Bacterial Agents Membrane chemistry 0210 nano-technology Antibacterial activity Bacteria Nuclear chemistry |
| Zdroj: | Carbohydrate polymers. 195 |
| ISSN: | 1879-1344 |
| Popis: | This work aims to synthesize poly(aminoethyl) modified chitin (PAEMC) and ascertain its antibacterial activity and mechanism. FTIR and 1H NMR results proved aminoethyl moieties were grafted to C6 OH and C3 OH on chitin backbone in the form of polymerization. XRD and TG/DTG analyses manifested its well-defined crystallinity and thermostability. PAEMC, with average molecular weight (MW) of 851.0 kDa, degree of deacetylation (DD) of 27.95%, and degree of substitution (DS) of 1.77, had good solubility in aqueous solutions over the pH range of 3–12, and also possessed high antimicrobial activity against Staphylococcus epidermidis, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Bacillus proteus, and Klebsiella pneumoniae, commonly causing chronic wound infections. Nucleic acid release, protein leakage, increased inner membrane permeability, and decreased cell surface hydrophobicity implied bacterial cytomembranes were substantially compromised in the presence of PAEMC. Microscopically, PAEMC visually perturbed bacteria, illustrating deformed and even collapsed morphologies. Overall, PAEMC possessed good solubility, effectively destroyed bacteria via a membrane damage mechanism, and might serve as an antibacterial agent for treatments of chronic wound infections. |
| Databáze: | OpenAIRE |
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