Corrigendum: A Novel Approach to Primary Cell Culture for Octopus Vulgaris Neurons
| Autor: | Anna Di Cosmo, Gianluca Polese, Naweed I. Syed, Fenglian Xu, Valeria Maselli |
|---|---|
| Přispěvatelé: | Maselli, V., Xu, F., Syed, N. I., Polese, G., Di Cosmo, A. |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Nervous system marine invertebrates Physiology medicine.medical_treatment Central nervous system ved/biology.organism_classification_rank.species Sensory system Biology cephalopods lcsh:Physiology Cephalopod Optic lobe 03 medical and health sciences Octopus 0302 clinical medicine Physiology (medical) biology.animal medicine optic lobes Model organism Original Research primary neuron cell culture lcsh:QP1-981 ved/biology Neurogenesis Marine invertebrate axon regeneration Correction Octopus vulgaris central nervous system Octopus vulgari 030104 developmental biology medicine.anatomical_structure Neuron Axotomy vertical-superior frontal system Neuroscience 030217 neurology & neurosurgery |
| Zdroj: | Frontiers in Physiology Frontiers in Physiology, Vol 9 (2018) |
| ISSN: | 1664-042X |
| Popis: | Octopus vulgaris is a unique model system for studying complex behaviors in animals. It has a large and centralized nervous system made up of lobes that are involved in controlling various sophisticated behaviors. As such, it may be considered as a model organism for untangling the neuronal mechanisms underlying behaviors—including learning and memory. However, despite considerable efforts, Octopus lags behind its other counterparts vis-à-vis its utility in deciphering the cellular, molecular and synaptic mechanisms underlying various behaviors. This study represents a novel approach designed to establish a neuronal cell culture protocol that makes this species amenable to further exploitation as a model system. Here we developed a protocol that enables dissociation of neurons from two specific Octopus' brain regions, the vertical-superior frontal system and the optic lobes, which are involved in memory, learning, sensory integration and adult neurogenesis. In particular, cells dissociated with enzyme papain and cultured on Poly-D-Lysine-coated dishes with L15-medium and fetal bovine serum yielded high neuronal survival, axon growth, and re-growth after injury. This model was also explored to define optimal culture conditions and to demonstrate the regenerative capabilities of adult Octopus neurons after axotomy. This study thus further underscores the importance of Octopus neurons as a model system for deciphering fundamental molecular and cellular mechanism of complex brain function and underlying behaviors. |
| Databáze: | OpenAIRE |
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