Yeast-Expressed Bacteriophage-Like Particles for the Packaging of Nanomaterials
| Autor: | Janis Freivalds, Tatyana Voronkova, Velta Ose, Andris Kazaks, Svetlana Kotelovica, Kaspars Tars |
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| Rok vydání: | 2013 |
| Předmět: |
viruses
Saccharomyces cerevisiae Bioengineering complex mixtures Applied Microbiology and Biotechnology Biochemistry Antiporters Pichia Pichia pastoris Bacteriophage chemistry.chemical_compound Bacteriophages Amino Acid Sequence Vaccines Virus-Like Particle Molecular Biology biology Virus Assembly RNA biology.organism_classification Molecular biology Yeast chemistry Nucleic acid Nanoparticles RNA Viral Capsid Proteins Heterologous expression DNA Biotechnology |
| Zdroj: | Molecular Biotechnology. 56:102-110 |
| ISSN: | 1559-0305 1073-6085 |
| DOI: | 10.1007/s12033-013-9686-0 |
| Popis: | Virus-like particles (VLPs) generated by heterologous expression of viral structural genes have become powerful tools in vaccine development. Recently, we and others have reported on the assembly of VLPs of the RNA bacteriophages MS2, Qβ, and GA in yeast. Here, we investigate the formation of VLPs of five additional phages in the yeasts Saccharomyces cerevisiae and Pichia pastoris, namely, the coliphages SP and fr, Acinetobacter phage AP205, Pseudomonas phage PP7, and Caulobacter phage φCb5. In all cases except SP, particle formation was detected, although VLP outcome varied from 0.2 to 8 mg from 1 g of wet cells. We have found that phage φCb5 VLPs easily dissociate into coat protein dimers when applied to strong anion exchangers. Upon salt removal and the addition of nucleic acid or its mimics and calcium ions, the dimers re-assemble into VLPs with high efficiency. A variety of compounds, including RNA, DNA, and gold nanoparticles can be packaged inside φCb5 VLPs. The ease with which phage φCb5 coat protein dimers can be purified in high quantities and re-assembled into VLPs makes them attractive for downstream applications including the internal packaging of nanomaterials and the chemical coupling of peptides of interest on the surface. |
| Databáze: | OpenAIRE |
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