The CHEK2 del5395 is a founder mutation without direct effects for cancer risk in the latvian population
| Autor: | E. Vjaters, J. Plonis, Dagnija Kalniete, Janis Gardovskis, Edvins Miklasevics, Miki Nakazawa-Miklasevica, Arvids Irmejs |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Oncology
medicine.medical_specialty checkpoint kinase 2 (chek2) gene Colorectal cancer Population QH426-470 03 medical and health sciences Prostate cancer Breast cancer Internal medicine Genetics medicine cancer education CHEK2 Genetics (clinical) 030304 developmental biology 0303 health sciences education.field_of_study business.industry chernobyl disaster liquidators 030305 genetics & heredity Cancer Odds ratio medicine.disease del5395 business Ovarian cancer |
| Zdroj: | Balkan Journal of Medical Genetics, Vol 18, Iss 2, Pp 33-36 (2015) |
| ISSN: | 1311-0160 |
| DOI: | 10.1515/bjmg-2015-0083 |
| Popis: | Our objective was to determine: 1) whether the checkpoint kinase 2 (CHEK2) del5395 (g.27417113-27422508 del, NC_000022.11) is a founder mutation in the Latvian population, 2) if there is an association between CHEK2 del5395 mutation and cancer risk, and 3) and whether the CHEK2 del5395 mutation impacts cancer predisposition in Chernobyl disaster liquidators (the civil and military personnel who were called upon to deal with consequences of the 1986 nuclear disaster) as well as geriatric populations. We recruited 438 breast cancer patients, 568 colorectal cancer patients, 399 ovarian cancer patients, 419 prostate cancer patients, 526 healthy blood donors, 480 Chernobyl disaster liquidators and 444 geriatric cancer-free participants. DNA samples were isolated from blood samples and subjected to multiplex polymerase chain reaction (PCR). The truncation of del5395 was estimated by fragment size of the multiplex PCR.All groups were compared to the healthy blood donors using Fisher’s exact test. All p values were two-sided and the odds ratios (OR) calculated by two-by-two table. In cancer groups, the del5395 mutation was most frequently observed in the ovarian cancer group (1.00%, OR = 1.32). In control groups, the del5395 mutation was most frequent (0.76%) in the healthy donors, which exceeded its frequency in the Chernobyl liquidators group and the geriatric group by 0.01 and 0.08%, respectively. For all groups, the OR appeared to be >1 only in ovarian cancer patients. However, OR rates showed no statistical significance in either cancer or control groups, with the p value fluctuating within the range of 0.39-1.00. The CHEK2 gene del5395 is a founder mutation in the Latvian population, which, however, does not have a direct impact on genetic predisposition toward colorectal, breast, ovarian and prostate cancer. |
| Databáze: | OpenAIRE |
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