Leptin and insulin downregulate leptin receptor gene expression in chicken-derived leghorn male hepatoma cells
| Autor: | Mohammed Taouis, Sami Dridi, S Cassy, Michel Derouet, Sabine Crochet |
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| Přispěvatelé: | Unité de Recherches Avicoles (URA), Institut National de la Recherche Agronomique (INRA), Laboratoire de Biologie Cellulaire et Moléculaire, Biotechnologies, INRA |
| Rok vydání: | 2003 |
| Předmět: |
Leptin
[SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology medicine.medical_treatment Adipose tissue MESH: Triiodothyronine Dexamethasone MESH: Recombinant Proteins 0302 clinical medicine MESH: Liver Neoplasms MESH: Reverse Transcriptase Polymerase Chain Reaction Gene expression Tumor Cells Cultured Insulin MESH: Animals MESH: Carcinoma Hepatocellular Receptor MESH: Receptors Cell Surface 0303 health sciences [SDV.NEU.PC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Psychology and behavior Reverse Transcriptase Polymerase Chain Reaction Liver Neoplasms digestive oral and skin physiology [SDV.NEU.SC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Cognitive Sciences General Medicine MESH: Gene Expression Regulation Recombinant Proteins MESH: Dexamethasone Lipogenesis Receptors Leptin Triiodothyronine hormones hormone substitutes and hormone antagonists medicine.medical_specialty Carcinoma Hepatocellular Blotting Western Receptors Cell Surface 030209 endocrinology & metabolism MESH: Receptors Leptin MESH: Insulin MESH: Poultry Diseases Biology 03 medical and health sciences Downregulation and upregulation Internal medicine medicine Animals MESH: Blotting Western RNA Messenger MESH: Tumor Cells Cultured Poultry Diseases MESH: RNA Messenger 030304 developmental biology Leptin receptor MESH: Leptin Endocrinology Gene Expression Regulation Animal Science and Zoology |
| Zdroj: | British Poultry Science British Poultry Science, Taylor & Francis, 2003, 82 (10), pp.1573-1579. ⟨10.1093/ps/82.10.1573⟩ |
| ISSN: | 0032-5791 0007-1668 1466-1799 |
| DOI: | 10.1093/ps/82.10.1573 |
| Popis: | International audience; In chickens, leptin is expressed mainly in the liver, where its receptor gene expression has also been reported, and in adipose tissue. In view of the key role played by the liver in lipogenesis in avian species, the hepatic expression of leptin may have physiological significance. In this study, we showed that leptin is constitutively expressed and secreted in a chicken-derived hepatoma cell line (LMH). Although insulin regulates leptin expression in vivo, incubation of LMH cells in the presence of 100 nM insulin for 24 or 48 h had no effect on leptin expression or its secretion in the culture medium. In addition, we developed a specific chicken leptin receptor real-time reverse transcription (RT)-PCR, and downregulation of leptin receptor gene expression by homologous and heterologous signals was demonstrated, as relative leptin receptor mRNA levels were significantly decreased after exposure of LMH cells to recombinant chicken leptin or porcine insulin. In conclusion, our results indicate that leptin is probably able to desensitize its own response in the chicken liver. Finally, the ability of insulin and leptin to regulate chicken leptin receptor gene expression suggests a direct role of leptin in the control of hepatic metabolism. |
| Databáze: | OpenAIRE |
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