Role of cytokine and neurotrophic factors in nicotine addiction in the conditioned place preference paradigm
| Autor: | João Luís Vieira Monteiro de Barros, Bruna da Silva Oliveira, Laila Asth, Fabrício A. Moreira, Antônio Lúcio Teixeira, Caroline Amaral Machado, Aline Silva de Miranda, Eliana Cristina de Brito Toscano, Magda Luciana de Paula Rosa |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Male
Nicotine medicine.medical_treatment Interleukin-1beta Prefrontal Cortex Context (language use) Striatum Pharmacology Hippocampus Mice Reward Neurotrophic factors Conditioning Psychological Glial cell line-derived neurotrophic factor Animals Humans Medicine Glial Cell Line-Derived Neurotrophic Factor Neuroinflammation biology business.industry Brain-Derived Neurotrophic Factor General Neuroscience Tobacco Use Disorder Corpus Striatum Conditioned place preference Interleukin-10 Disease Models Animal Cytokine Neuroinflammatory Diseases biology.protein business Injections Intraperitoneal medicine.drug |
| Zdroj: | Neuroscience Letters. 764:136235 |
| ISSN: | 0304-3940 |
| DOI: | 10.1016/j.neulet.2021.136235 |
| Popis: | The mechanisms involved in the maintenance of cigarette smoking and nicotine reward remain unclear. Immune response might play an important role in this context. Nicotine may induce both central and systemic inflammatory responses as well as changes in the regulation of brain-derived neurotrophic factor (BDNF). The conditioned place preference (CPP) is a method used for the evaluation of nicotine-induced reward, reproducing nicotine-seeking behavior in humans. So far, there are no studies investigating the relationship between neuroinflammation and nicotine-induced CPP. This study aimed to evaluate the levels of inflammatory mediators and neurotrophic factors in key areas of the central nervous system (CNS) of mice subject to nicotine-induced CPP. CPP was induced with an intraperitoneal administration of 0.5 mg/kg of nicotine in male Swiss mice, using an unbiased protocol. Control group received vehicle by the same route. The levels of cytokines, chemokines, and neurotrophic factors were measured using Enzyme-Linked Immunosorbent Assay (ELISA) in the brain after CPP test. As expected, nicotine induced place preference behavior. In parallel, we observed increased peripheral levels of IL-6 and IL-10 alongside increased hippocampal levels of NGF but decreased GDNF in mice treated with nicotine compared to controls. In the striatum, nicotine promoted decrease of IL-1s, IL-10 and GDNF levels, while the levels of all the mediators were similar between groups in the pre-frontal cortex. Our results provide evidence on the role of cytokines and neurotrophic factors in nicotine-induced CPP in mice. |
| Databáze: | OpenAIRE |
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