High STRN Expression Promotes HCC Invasion and Migration but Not Cell Proliferation or Apoptosis through Facilitating Epithelial-Mesenchymal Transition

Autor: Jing-Hao Yao, Ling-Jie Xu, Qian-Yu Du, Yan Yang, Fu-You Zhao, Yong-Chun Zhou
Rok vydání: 2019
Předmět:
0301 basic medicine
Genetic Markers
Carcinoma
Hepatocellular
Epithelial-Mesenchymal Transition
Article Subject
Cell
Vimentin
Apoptosis
Nerve Tissue Proteins
Biology
General Biochemistry
Genetics and Molecular Biology
03 medical and health sciences
0302 clinical medicine
Downregulation and upregulation
Antigens
CD
Cell Line
Tumor
medicine
Biomarkers
Tumor
Humans
Epithelial–mesenchymal transition
Gene Silencing
Cell Proliferation
Gene knockdown
General Immunology and Microbiology
Cell growth
Liver Neoplasms
Membrane Proteins
General Medicine
Hep G2 Cells
Cadherins
digestive system diseases
Gene Expression Regulation
Neoplastic
030104 developmental biology
medicine.anatomical_structure
Cell culture
030220 oncology & carcinogenesis
Cancer research
biology.protein
Medicine
Calmodulin-Binding Proteins
Research Article
Zdroj: BioMed Research International
BioMed Research International, Vol 2020 (2020)
ISSN: 2314-6141
Popis: A STRN-ALK fusion protein has been recently identified as a potential therapeutic target in multiple cancers; however, the role of STRN alone in regulating the biological function of hepatocellular carcinoma (HCC) remains unclear. In this study, we firstly detected an overexpression of STRN in HCC tissues compared to that in adjacent nontumour (ANT) tissues through IHC analysis, and the expression level of this protein was positively correlated with lymph node metastasis and TNM stage. In vitro, high expression of STRN was also confirmed in different HCC cell lines, and regulation of STRN expression in Huh7 cells did not significantly affect tumour cell proliferation or apoptosis but was positively correlated with tumour cell invasion and migration capacities. Moreover, both the knockdown and overexpression of STRN in Huh7 cells can lead to cell morphological changes that are accompanied with an alteration of epithelial-mesenchymal transition (EMT) molecular markers E-cadherin and Vimentin. Finally, STRN was further proved to be negatively related to E-cadherin expression but positively related to Vimentin expression in human HCC tissue samples. Taken together, STRN is upregulated in HCC and acts as a tumour promoter regulating cell invasion and migration through facilitating the EMT process.
Databáze: OpenAIRE
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