Occurrence of enterovirus RNA in serum of children with newly diagnosed type 1 diabetes and islet cell autoantibody-positive subjects in a population with a low incidence of type 1 diabetes
| Autor: | Eduardo Cabrera-Rode, Luis Sarmiento, Pedro Gonzalez, Ileana Cuba, Lai Heng-Hung, Oscar Diaz-Horta, Gisela Molina, Pedro Mas-Lago, Lister Lekuleni, Magile Fonseca, Abel Diaz Borroto |
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| Rok vydání: | 2007 |
| Předmět: |
Male
medicine.medical_specialty Diabetic ketoacidosis Adolescent Immunology Population Biology medicine.disease_cause Gastroenterology Diabetic Ketoacidosis Internal medicine Insulin-Secreting Cells medicine Enterovirus Infections Immunology and Allergy Humans First-degree relatives education Child Autoantibodies Enterovirus Type 1 diabetes geography education.field_of_study geography.geographical_feature_category Incidence (epidemiology) Incidence Autoantibody Cuba Infant medicine.disease Islet Europe Endocrinology Diabetes Mellitus Type 1 Child Preschool RNA Viral Female |
| Zdroj: | Autoimmunity. 40(7) |
| ISSN: | 1607-842X |
| Popis: | The penetrance of type 1 diabetes mellitus (T1DM) in a genetically susceptible population is largely determined by environmental influences amongst which the human enteroviruses are prominent putative factors.The aim of this study was to determine the occurrence of enterovirus RNA in serum of children with type 1 diabetes at onset and ICA-positive subjects in a population with low incidence of type 1 diabetes and high circulation of enteroviruses.Serum samples were collected from children with newly diagnosed type 1 diabetes (n = 34); islet autoantibody-positive (n = 32) and -negative (n = 31) first-degree relatives of type 1 diabetic patients; and control subjects (n = 194). Enteroviral RNA was assessed using a highly sensitive reverse transcriptase-polymerase chain reaction method.The frequency of positive signals corresponding to enteroviral sequence amplifications were higher in newly diagnosed T1DM children (9/34, 26.5%) and islet autoantibody-positive first-degree relatives (5/32, 15.6%) than in their corresponding matched controls (2/68, 2.9%, p = 0.0007 and 0/64, 0.0%, p = 0.0033, respectively). The presence of enteroviral RNA appeared to be associated with severe diabetic ketoacidosis at onset (pH7.1, p = 0.0328) and high ICA titres (or = 20 JDF units, p0.05).Despite there is a high circulation of enteroviruses and a low type 1 diabetes incidence in the Cuban population, the presence of enteroviral RNA is associated with type 1 diabetes and beta-cell autoimmunity and is similar to European countries in which this scenario is reversed. |
| Databáze: | OpenAIRE |
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