Down-Regulation of Hypoxia-Inducible Factor-1α and Downstream Glucose Transporter Protein-1 Gene by β-elemene Enhancing the Radiosensitivity of Lung Adenocarcinoma Transplanted Tumor
Autor: | Hui-En Wang, Wenfei Xue, Qingtao Zhao, Xiaopeng Zhang, Zhonghui Hu, Guo-Chen Duan, Wenbo Wu, Hua Zhang, Lei Yu |
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Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
HIF-1α 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Downregulation and upregulation medicine Pharmacology (medical) Radiosensitivity transplanted tumor Original Research A549 cell β-elemene Glucose transporter GLUT-1 medicine.disease Transplantation 030104 developmental biology Oncology chemistry radiosensitivity 030220 oncology & carcinogenesis Cancer research Adenocarcinoma Immunohistochemistry Elemene |
Zdroj: | OncoTargets and therapy |
ISSN: | 1178-6930 |
Popis: | Purpose To study the effect of β-elemene on the radiosensitivity of A549 cell xenograft tumor and potential mechanisms by which β-elemene regulates the expression of hypoxia-inducible factor-1α (HIF-1α) and glucose transporter protein-1 (GLUT-1). Methods Using an A549 cell transplantation tumor model with male nude mice, we studied the effect of β-elemene on the radiosensitivity of non-small cell lung cancer (NSCLC). The expression of HIF-1α and GLUT-1 was detected by real-time PCR, Western blotting and immunohistochemistry. The relationship between the radiosensitivity of β-elemene and the expression of HIF-1α and GLUT-1 was analyzed. Results β-elemene and radiotherapy intervened in the growth of transplanted tumors in varying degrees. The enhancement factor (EF=2.44>1) was calculated; β-elemene at 45 mg/kg had the most significant enhanced effect on radiosensitivity. When β-elemene was used in combination with radiation, the expression of HIF-1α and GLUT-1 was significantly decreased, and there was a positive correlation between the two genes. Conclusion β-elemene exhibits a radiosensitizing effect on A549 cell xenograft tumor. The underlying molecular mechanism is probably associated with the down-regulation of HIF-1α and GLUT-1 expression, suggesting that β-elemene may directly or indirectly inhibit the expression of HIF-1α and GLUT-1. There is a positive significant correlation between expression of HIF-1α and GLUT-1. HIF-1α and downstream GLUT-1 could be used as a new target for the radiosensitization of NSCLC. |
Databáze: | OpenAIRE |
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