Relevance of epidermal growth factor to improve steatotic liver preservation in IGL-1 solution
| Autor: | Mohamed Amine Zaouali, María Calvo, H. Ben Abdennebi, Francisco A. García-Gil, I. Ben Mosbah, Joan Roselló-Catafau, Arnau Panisello, Susagna Padrissa-Altés, M. Bjaoui, Dalila Saidane-Mosbahi |
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| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
medicine.medical_specialty
Nitric Oxide Synthase Type III Blotting Western Preservation Biological medicine.disease_cause Proinflammatory cytokine Nitric oxide chemistry.chemical_compound Epidermal growth factor Internal medicine medicine Animals Transplantation Epidermal Growth Factor business.industry Fatty liver medicine.disease Rats Fatty Liver Solutions Oxidative Stress Endocrinology chemistry Biochemistry Surgery Steatosis business Reperfusion injury Oxidative stress |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 0041-1345 |
| Popis: | Aim Static preservation solution is critical for liver graft outcomes, especially when steatosis is present. Institut Georges Lopez (IGL)-1 solution protects fatty livers effectively against cold ischemia reperfusion injury. Its benefits are mediated by nitric oxide and prevention of oxidative stress. The supplementation of IGL-1 with epidermal growth factor (EGF) enhances steatotic graft preservation by increasing adenosine triphosphate content, thereby mitigating oxidative stress and mitochondrial damage. Methods After steatotic livers were preserved for 24 hours in IGL-1 solution with or without EGF supplements, they were perfused ex vivo for 2 hours at 37°C. The benefits of EGF were assessed by evidences of hepatic damage and functiontransaminases, bile production, and flow rateas well as by other factors presumably associated with the poor tolerance of fatty livers toward cold ischemia-reperfusion injury (IRI)energy metabolism, mitochondrial damage, oxidative stress, eNOS activity and proinflammatory interleukin (IL) beta content. Results Steatotic livers preserved in IGL-1 solutions supplemented with EGF (10 μg/L) showed lower transaminase levels, greater bile production, and ameliorated flow rates when compared to IGL-1 alone. In addition, energy metabolism deterioration, mitochondrial damage, oxidative stress, and cytokine IL-1 beta release were prevented. Conclusion EGF addition to IGL-1 increased fatty liver graft preservation, thereby reducing steatotic liver damage against cold IRI. © 2010 Elsevier Inc. This study was supported by the Ministerio de de Sanidad y Consumo (PI 081988), CIBER-ehd, Instituto Carlos III, Madrid and Ministerio de Asuntos Exteriores y de Cooperación Internacionales (A/020255/08 and A/02987/09), Madrid. Mohamed Amine Zaouali is fellowship-holder from the Societat Catalana de Trasplantament. |
| Databáze: | OpenAIRE |
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