Indirect competitive ELISA based on monoclonal antibody for the detection of 5-hydroxymethyl-2-furfural in milk, compared with HPLC
| Autor: | Hecheng Meng, Yongguang Guan, Xinlan Wu |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Mice
Inbred BALB C Chromatography medicine.drug_class Chemistry Antibodies Monoclonal Enzyme-Linked Immunosorbent Assay Monoclonal antibody Furfural Rapid detection High-performance liquid chromatography Absorbance Standard curve chemistry.chemical_compound Mice Milk Genetics medicine Animals Animal Science and Zoology Female Furaldehyde Haptens 5 hydroxymethyl 2 furfural Chromatography High Pressure Liquid Food Science |
| Zdroj: | Journal of dairy science. 96(8) |
| ISSN: | 1525-3198 |
| Popis: | In this study, a method for rapid detection of 5-hydroxymethyl-2-furfural (HMF) was investigated. Monoclonal antibody (anti-HMF) was prepared and evaluated by an indirect competitive ELISA (ic-ELISA) format. The optimized standard curve was y=-0.2097x+1.0432 [where x is the logarithm (base 10) of the values of the HMF concentration and y is the absorbance of ic-ELISA results tested at 490 nm] and the linear detection range was 0.008 to 32.768 mg/L. The percentage of cross-reactivity of HMF with 5 major furfural derivatives was less than 2.92%. Finally, the established ic-ELISA format was used to test HMF in milk, and compared with the result obtained by HPLC, which produced an error of about 0.3%. Based on the data in this experiment, we concluded that the established ic-ELISA format was reliable with a high specificity. |
| Databáze: | OpenAIRE |
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