Preliminary report of in vitro and in vivo effectiveness of dornase alfa on SARS-CoV-2 infection
| Autor: | Cihan Tastan, Sevda Demir, Ercument Ovali, M. Kara, Utku Seyis, Derya Dilek Kancagi, Gozde Sir Karakus, Rehile Zengin, Caglar Cuhadaroglu, N.B. Pelit, Nur Birgen, E. Deliceo, Selen Abanuz, Cansu Hemsinlioglu, Ayse Sesin Kocagoz, H.K. Okur, Koray Yalcin, Bulut Yurtsever, M.E. Yildiz |
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| Přispěvatelé: | Acibadem University Dspace |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
ARDS 030106 microbiology dornase alfa neutrophil extracellular traps Microbiology Cystic fibrosis lcsh:Infectious and parasitic diseases 03 medical and health sciences medicine lcsh:RC109-216 Respiratory system Lung Respiratory tract infections Coronavirus disease 2019 business.industry NETosis Dornase alfa medicine.disease Pneumonia 030104 developmental biology Infectious Diseases medicine.anatomical_structure Immunology business First Clinical Case Report medicine.drug Respiratory tract severe acute respiratory syndrome coronavirus 2 |
| Zdroj: | New Microbes and New Infections, Vol 37, Iss, Pp 100756-(2020) New Microbes and New Infections |
| ISSN: | 2052-2975 |
| Popis: | Dornase alfa, the recombinant form of the human DNase I enzyme, breaks down neutrophil extracellular traps (NET) that include a vast amount of DNA fragments, histones, microbicidal proteins and oxidant enzymes released from necrotic neutrophils in the highly viscous mucus of cystic fibrosis patients. Dornase alfa has been used for decades in patients with cystic fibrosis to reduce the viscoelasticity of respiratory tract secretions, to decrease the severity of respiratory tract infections, and to improve lung function. Previous studies have linked abnormal NET formations to lung diseases, especially to acute respiratory distress syndrome (ARDS). It is well known that novel coronavirus disease 2019 (COVID-19) pneumonia progresses to ARDS and even multiple organ failure. High blood neutrophil levels are an early indicator of COVID-19 and predict severe respiratory diseases. Also it is reported that mucus structure in COVID-19 is very similar to that in cystic fibrosis due to the accumulation of excessive NET in the lungs. In this study, we showed the recovery of three individuals with COVID-19 after including dornase alfa in their treatment. We followed clinical improvement in the radiological analysis (two of three cases), oxygen saturation (SpO(2)), respiratory rate, disappearance of dyspnoea, coughing and a decrease in NET formation and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral load after the treatment. Also here, we share our preliminary results suggesting that dornase alfa has an anti-viral effect against SARS-CoV-2 infection in a green monkey kidney cell line, Vero, and a bovine kidney cell line, MDBK, without determined cytotoxicity on healthy peripheral blood mononuclear cells. (C) 2020 The Authors. Published by Elsevier Ltd. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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