Biorheology of Platelet Activation in the Bloodstream Distal to Thrombus Formation
| Autor: | Sandra M. Baker-Groberg, Asako Itakura, Christoph Gerdes, Jeevan Maddala, Owen J. T. McCarty, Andras Gruber, Ralf Vetter, Erik I. Tucker, Jevgenia Zilberman-Rudenko |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
biology Factor X 030204 cardiovascular system & hematology medicine.disease Article General Biochemistry Genetics and Molecular Biology Fibrin 03 medical and health sciences chemistry.chemical_compound Tissue factor 030104 developmental biology 0302 clinical medicine Thrombin chemistry Coagulation Biochemistry Modeling and Simulation biology.protein medicine Biophysics Platelet Platelet activation Thrombus medicine.drug |
| Zdroj: | Cellular and Molecular Bioengineering. 9:496-508 |
| ISSN: | 1865-5033 1865-5025 |
| Popis: | Thrombus growth at the site of vascular injury is mediated by the sequential events of platelet recruitment, activation and aggregation concomitant with the initiation of the coagulation cascade, resulting in local thrombin generation and fibrin formation. While the biorheology of a localized thrombus formation has been well studied, it is unclear whether local sites of thrombin generation propagate platelet activation within the bloodstream. In order to study the physical biology of platelet activation downstream of sites of thrombus formation, we developed a platform to measure platelet activation and microaggregate formation in the bloodstream. Our results show that thrombi formed on collagen and tissue factor promote activation and aggregation of platelets in the bloodstream in a convection-dependent manner. Pharmacological inhibition of the coagulation factors (F) X, XI or thrombin dramatically reduced the degree of distal platelet activation and microaggregate formation in the bloodstream without affecting the degree of local platelet deposition and aggregation on a surface of immobilized collagen. Herein we describe the development and an example of the utility of a platform to study platelet activation and microaggregate formation in the bloodstream (convection-limited regime) relative to the local site of thrombus formation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink