Natural co-infection of influenza A/H3N2 and A/H1N1pdm09 viruses resulting in a reassortant A/H3N2 virus
Autor: | Philippe Buchy, Paul F. Horwood, Srey Viseth Horm, Borann Sar, Philippe Dussart, Sovann Ly, Phalla Y, Savuth Chin, Sareth Rith |
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Přispěvatelé: | Institut Pasteur du Cambodge, Réseau International des Instituts Pasteur (RIIP), National Institute of Public Health [Phnom Penh, Cambodge], Centers for Disease Control and Prevention [Phnom Penh], Embassy of the United States of America, Ministry of Health [Phnom Penh], Unité de Virologie / Virology Unit [Phnom Penh], Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), The activities of the Cambodian National Influenza Centre at the Institut Pasteur in Cambodia were supported by theWorld Health Organization office in Cambodia. The activities of the National Institute of Public Health were supported by the Cambodian Ministry of Health and US CDC, Cooperative Agreement U51IP000522. |
Jazyk: | angličtina |
Předmět: |
Male
viruses Reassortment Genome Influenza A Virus H1N1 Subtype MESH: Phylogeny A/H3N2 Phylogeny 0303 health sciences Coinfection MESH: Influenza Human virus diseases H3N2 3. Good health Co-infection Infectious Diseases MESH: Reassortant Viruses Child Preschool [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology MESH: Genome Viral Cambodia Viral load Influenza Seasonal Co-infection Reassortant Viruses Short Communication A/H1N1pdm09 Genome Viral Biology MESH: Influenza A Virus H3N2 Subtype complex mixtures Virus MESH: Influenza A Virus H1N1 Subtype 03 medical and health sciences Phylogenetics Virology Influenza Human medicine Humans Gene 030304 developmental biology Seasonal MESH: Humans Reassortant 030306 microbiology H1N1pdm09 MESH: Cambodia Influenza A Virus H3N2 Subtype MESH: Child Preschool medicine.disease MESH: Male Influenza MESH: Coinfection respiratory tract diseases |
Zdroj: | Journal of Clinical Virology Journal of Clinical Virology, Elsevier, 2015, 73, pp.108-111. ⟨10.1016/j.jcv.2015.11.008⟩ |
ISSN: | 1386-6532 |
DOI: | 10.1016/j.jcv.2015.11.008 |
Popis: | Highlights • A co-infection between influenza A/H3N2 and A/H1N1pdm09 was detected from a patient. • A reassortant A/H3N2 virus was isolated with a NS1 gene from A/H1N1pdm09. • The reassortant virus was viable and able to replicate in cell culture. Background Despite annual co-circulation of different subtypes of seasonal influenza, co-infections between different viruses are rarely detected. These co-infections can result in the emergence of reassortant progeny. Study design We document the detection of an influenza co-infection, between influenza A/H3N2 with A/H1N1pdm09 viruses, which occurred in a 3 year old male in Cambodia during April 2014. Both viruses were detected in the patient at relatively high viral loads (as determined by real-time RT-PCR CT values), which is unusual for influenza co-infections. As reassortment can occur between co-infected influenza A strains we isolated plaque purified clonal viral populations from the clinical material of the patient infected with A/H3N2 and A/H1N1pdm09. Results Complete genome sequences were completed for 7 clonal viruses to determine if any reassorted viruses were generated during the influenza virus co-infection. Although most of the viral sequences were consistent with wild-type A/H3N2 or A/H1N1pdm09, one reassortant A/H3N2 virus was isolated which contained an A/H1N1pdm09 NS1 gene fragment. The reassortant virus was viable and able to infect cells, as judged by successful passage in MDCK cells, achieving a TCID50 of 104/ml at passage number two. There is no evidence that the reassortant virus was transmitted further. The co-infection occurred during a period when co-circulation of A/H3N2 and A/H1N1pdm09 was detected in Cambodia. Conclusions It is unclear how often influenza co-infections occur, but laboratories should consider influenza co-infections during routine surveillance activities. |
Databáze: | OpenAIRE |
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