Natural co-infection of influenza A/H3N2 and A/H1N1pdm09 viruses resulting in a reassortant A/H3N2 virus

Autor: Philippe Buchy, Paul F. Horwood, Srey Viseth Horm, Borann Sar, Philippe Dussart, Sovann Ly, Phalla Y, Savuth Chin, Sareth Rith
Přispěvatelé: Institut Pasteur du Cambodge, Réseau International des Instituts Pasteur (RIIP), National Institute of Public Health [Phnom Penh, Cambodge], Centers for Disease Control and Prevention [Phnom Penh], Embassy of the United States of America, Ministry of Health [Phnom Penh], Unité de Virologie / Virology Unit [Phnom Penh], Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), The activities of the Cambodian National Influenza Centre at the Institut Pasteur in Cambodia were supported by theWorld Health Organization office in Cambodia. The activities of the National Institute of Public Health were supported by the Cambodian Ministry of Health and US CDC, Cooperative Agreement U51IP000522.
Jazyk: angličtina
Předmět:
Male
viruses
Reassortment
Genome
Influenza A Virus
H1N1 Subtype
MESH: Phylogeny
A/H3N2
Phylogeny
0303 health sciences
Coinfection
MESH: Influenza
Human
virus diseases
H3N2
3. Good health
Co-infection
Infectious Diseases
MESH: Reassortant Viruses
Child
Preschool
[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology
MESH: Genome
Viral
Cambodia
Viral load
Influenza Seasonal Co-infection
Reassortant Viruses
Short Communication
A/H1N1pdm09
Genome
Viral
Biology
MESH: Influenza A Virus
H3N2 Subtype
complex mixtures
Virus
MESH: Influenza A Virus
H1N1 Subtype
03 medical and health sciences
Phylogenetics
Virology
Influenza
Human
medicine
Humans
Gene
030304 developmental biology
Seasonal
MESH: Humans
Reassortant
030306 microbiology
H1N1pdm09
MESH: Cambodia
Influenza A Virus
H3N2 Subtype
MESH: Child
Preschool
medicine.disease
MESH: Male
Influenza
MESH: Coinfection
respiratory tract diseases
Zdroj: Journal of Clinical Virology
Journal of Clinical Virology, Elsevier, 2015, 73, pp.108-111. ⟨10.1016/j.jcv.2015.11.008⟩
ISSN: 1386-6532
DOI: 10.1016/j.jcv.2015.11.008
Popis: Highlights • A co-infection between influenza A/H3N2 and A/H1N1pdm09 was detected from a patient. • A reassortant A/H3N2 virus was isolated with a NS1 gene from A/H1N1pdm09. • The reassortant virus was viable and able to replicate in cell culture.
Background Despite annual co-circulation of different subtypes of seasonal influenza, co-infections between different viruses are rarely detected. These co-infections can result in the emergence of reassortant progeny. Study design We document the detection of an influenza co-infection, between influenza A/H3N2 with A/H1N1pdm09 viruses, which occurred in a 3 year old male in Cambodia during April 2014. Both viruses were detected in the patient at relatively high viral loads (as determined by real-time RT-PCR CT values), which is unusual for influenza co-infections. As reassortment can occur between co-infected influenza A strains we isolated plaque purified clonal viral populations from the clinical material of the patient infected with A/H3N2 and A/H1N1pdm09. Results Complete genome sequences were completed for 7 clonal viruses to determine if any reassorted viruses were generated during the influenza virus co-infection. Although most of the viral sequences were consistent with wild-type A/H3N2 or A/H1N1pdm09, one reassortant A/H3N2 virus was isolated which contained an A/H1N1pdm09 NS1 gene fragment. The reassortant virus was viable and able to infect cells, as judged by successful passage in MDCK cells, achieving a TCID50 of 104/ml at passage number two. There is no evidence that the reassortant virus was transmitted further. The co-infection occurred during a period when co-circulation of A/H3N2 and A/H1N1pdm09 was detected in Cambodia. Conclusions It is unclear how often influenza co-infections occur, but laboratories should consider influenza co-infections during routine surveillance activities.
Databáze: OpenAIRE
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