A sensitive assay for rapid detection and quantification of aphanomyces euteiches in soil
| Autor: | Marc-Antoine Cannesan, Azeddine Driouich, Lisa Castel, Maïté Vicré-Gibouin, Karine Laval, Anne Moussart, Isabelle Trinsoutrot-Gattin, Mélanie Bressan, Christophe Gangneux |
|---|---|
| Přispěvatelé: | École supérieure d'ingénieurs et de techniciens pour l'agriculture (ESITPA), Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale (Glyco-MEV), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU), Institut de Génétique, Environnement et Protection des Plantes (IGEPP), AGROCAMPUS OUEST-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche Agronomique (INRA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Institut National de la Recherche Agronomique (INRA)-Université de Rennes (UR)-AGROCAMPUS OUEST |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
[SDV]Life Sciences [q-bio]
Aphanomyces Plant Science Real-Time Polymerase Chain Reaction Plant Roots complex mixtures DNA Ribosomal Spacer Root rot Soil Microbiology ComputingMilieux_MISCELLANEOUS Plant Diseases 2. Zero hunger Oomycete biology Peas Reproducibility of Results food and beverages Pesticide biology.organism_classification Agronomy Soil water Oospore France Aphanomyces euteiches Agronomy and Crop Science Soil microbiology |
| Zdroj: | Phytopathology Phytopathology, American Phytopathological Society, 2014, 104 (10), pp.1138-1147. ⟨10.1094/PHYTO-09-13-0265-R⟩ Phytopathology, 2014, 104 (10), pp.1138-1147. ⟨10.1094/PHYTO-09-13-0265-R⟩ |
| ISSN: | 0031-949X |
| Popis: | International audience; Aphanomyces euteiches is a widespread oomycete pathogen causing root rot in a wide range of leguminous crops. Losses can reach up to 100% for pea culture and there is currently no registered pesticide for its control. Crop management remains the most efficient tool to control root rot, and avoidance of infested soil seems to be the optimal solution. A test was developed to identify fields suitable for pea crops, consisting of the determination of the inoculum potential of soil using baiting plants. A new rapid, specific, and sensitive molecular method is described allowing the quantification of less than 10 oospores per gram of soil. This challenge is achieved by a real-time polymerase chain reaction procedure targeting internal transcribed spacer 1 from the ribosomal DNA operons. A preliminary study based on typical soils from northwestern France demonstrated that the A. euteiches oospore density in soil is related to the inoculum potential. Furthermore, this method has proved sensitive enough to accurately study the influence of biotic factors that may govern the actual emergence of root rot. |
| Databáze: | OpenAIRE |
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