MINIMUM NUMBER OF ISLETS REQUIRED TO MAINTAIN EUGLYCEMIA AND THEIR REDUCED IMMUNOGENICITY AFTER TRANSPLANTATION INTO DIABETIC MICE
| Autor: | Takashi Maki, Eduard Montana, Porter J, Anthony P. Monaco, Hiroki Ohzato |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Blood Glucose
Male endocrine system medicine.medical_specialty endocrine system diseases Ratón medicine.medical_treatment Islets of Langerhans Transplantation Mice Inbred Strains Diabetes Mellitus Experimental Islets of Langerhans Mice Internal medicine Diabetes mellitus medicine Animals Insulin Mice Inbred C3H Transplantation Glucose tolerance test geography geography.geographical_feature_category medicine.diagnostic_test business.industry Immunogenicity Graft Survival Glucose Tolerance Test Islet medicine.disease Mice Inbred C57BL Streptozocin Endocrinology business |
| Zdroj: | Europe PubMed Central |
| ISSN: | 0041-1337 |
| DOI: | 10.1097/00007890-199308000-00003 |
| Popis: | In streptozocin (SZ)-induced diabetic mice, 200 islets, but not 50 islets, consistently restore euglycemia within 1 week of transplantation. To determine the minimum number of islets sufficient to maintain euglycemia in a diabetic mouse, we first transplanted 50 and 150 syngeneic islets simultaneously into the right (RK) and left kidney (LK), respectively, and then removed the LK 1 week post-transplantation. The remaining 50 islets maintained euglycemia in 8 of 11 mice with normal intravenous glucose tolerance tests (IVGTT). Protection of 50 islets for at least 7 days was necessary because removal of the 150 islets at 5 or 3 days resulted in a much lower incidence of persistent euglycemia. Similarly, 25 islets were capable of maintaining euglycemia in 2 of 9 mice once hyperglycemia was reversed by split-transplantation of 25 (RK) and 175 (LK) islets. To examine if 50-islet allografts survive longer than 200-islet allografts, we split-transplanted 50 DBA/2 islets in the RK and 150 islets of either B6 (syngeneic), DBA/2 (allogeneic), or C3H/He (third party allogeneic) mouse origin in the LK in 3 groups of diabetic C57BL/6 (B6) mice. The survival of 50 DBA/2 islets in each group after removal of the LK on day 7 was compared to that of 200 DBA/2 islets in control B6 mice. Maximum prolongation of allograft survival was obtained with 50 DBA/2 islets that were split-transplanted with syngeneic B6 islets. These results clearly demonstrate that 50 islets are sufficient to maintain normal glucose tolerance once euglycemia is induced by transplantation of a larger number (i.e., 200) of islets and that 50 islet allografts are much less immunogenic than 200-islet allografts. |
| Databáze: | OpenAIRE |
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