The insulin receptor substrate Chico regulates antibacterial immune function in Drosophila
| Autor: | Sarah McCormack, Ioannis Eleftherianos, Shruti Yadav, Eric Kenney, Dustin Cooper, Upasana Shokal |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Aging Immunology Mutant Chico Long-lived mutant Microbiology 03 medical and health sciences Immune system Immunity Insulin receptor substrate Photorhabdus luminescens Melanogaster Innate immunity Innate immune system biology Research fungi biology.organism_classification Ageing Drosophila melanogaster 030104 developmental biology Insect pathogen Infection Photorhabdus |
| Zdroj: | Immunity & Ageing : I & A |
| ISSN: | 1742-4933 |
| DOI: | 10.1186/s12979-016-0072-1 |
| Popis: | Background Molecular and genetic studies in model organisms have recently revealed a dynamic interplay between immunity and ageing mechanisms. In the fruit fly Drosophila melanogaster, inhibition of the insulin/insulin-like growth factor signaling pathway prolongs lifespan, and mutations in the insulin receptor substrate Chico extend the survival of mutant flies against certain bacterial pathogens. Here we investigated the immune phenotypes, immune signaling activation and immune function of chico mutant adult flies against the virulent insect pathogen Photorhabdus luminescens as well as to non-pathogenic Escherichia coli bacteria. Results We found that D. melanogaster chico loss-of-function mutant flies were equally able to survive infection by P. luminescens or E. coli compared to their background controls, but they contained fewer numbers of bacterial cells at most time-points after the infection. Analysis of immune signaling pathway activation in flies infected with the pathogenic or the non-pathogenic bacteria showed reduced transcript levels of antimicrobial peptide genes in the chico mutants than in controls. Evaluation of immune function in infected flies revealed increased phenoloxidase activity and melanization response to P. luminescens and E. coli together with reduced phagocytosis of bacteria in the chico mutants. Changes in the antibacterial immune function in the chico mutants was not due to altered metabolic activity. Conclusions Our results indicate a novel role for chico in the regulation of the antibacterial immune function in D. melanogaster. Similar studies will further contribute to a better understanding of the interconnection between ageing and immunity and lead to the identification and characterization of the molecular host components that modulate both important biological processes. |
| Databáze: | OpenAIRE |
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