Effects of chronic unpredictable mild stress on ovarian reserve in female rats: Feasibility analysis of a rat model of premature ovarian failure
| Autor: | Xiao‑Ming Pan, Xiao‑Yan Fu, Ning Zhang, Haohao Chen, Yi‑Ping Lin, Wen‑Qian Wang, Ming‑Xing Ding, Qun Zheng, Yuan‑Shu Fang, Ying‑Er Qiu |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Anti-Mullerian Hormone endocrine system Cancer Research medicine.medical_specialty endocrine system diseases Gonadotropin-releasing hormone Primary Ovarian Insufficiency Biochemistry Open field Gonadotropin-Releasing Hormone 03 medical and health sciences 0302 clinical medicine Internal medicine Genetics medicine Animals Chronic stress Ovarian reserve Ovarian Reserve Molecular Biology Estrous cycle Estradiol business.industry Depression Ovary medicine.disease Molecular medicine female genital diseases and pregnancy complications Premature ovarian failure Rats Disease Models Animal 030104 developmental biology Endocrinology Oncology Molecular Medicine Female business hormones hormone substitutes and hormone antagonists 030217 neurology & neurosurgery Stress Psychological Hormone |
| Zdroj: | Molecular medicine reports. 18(1) |
| ISSN: | 1791-3004 |
| Popis: | Premature ovarian failure (POF) results from a number of disorders. The POF model is primarily based on chemotherapeutic injury, and hence is not suitable for assessing the effects of chronic stress on ovarian function. Therefore, improved animal models are required to analyze the effects of chronic stress on ovarian reserve. The feasibility of the chronic unpredictable mild stress (CUMS) method for establishing a model of POF was examined. The depressive behavior exhibited by rats was evaluated with the open field and sucrose preference tests. Vaginal smears were obtained for assessment of the estrous cycle. The ovarian reserve of the animals was evaluated using the estrous cycle, ovarian histology and serum levels of gonadotropin releasing hormone (GnRH), follicle‑stimulating hormone (FSH), estradiol (E2), and anti‑Mullerian hormone (AMH). Compared with the control group, body weight, time spent in the center, horizontal movement, vertical frequency, consumption of sucrose, sucrose preference, number of small follicles from the rats, and serum E2, AMH and GnRH levels were significantly decreased in the CUMS group (all P |
| Databáze: | OpenAIRE |
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