Characterization and genomic analysis of Exiguobacterium alkaliphilum B-3531D, an efficient crude oil degrading strain
| Autor: | Yulia Kocharovskaya, Viktor I. Solomentsev, Yanina Delegan, Angelika A. Sizova, Alina Zinovieva, Leila Iminova, Mikhail Goyanov, Nikita S. Lyakhovchenko, Alexander Bogun, Inna P. Solyanikova |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Genome assembly
biology Strain (chemistry) Chemistry Sodium Short Communication chemistry.chemical_element Biodegradation Exiguobacterium biology.organism_classification Applied Microbiology and Biotechnology Genome Crude oil Plasmid Environmental biotechnology Seawater Food science TP248.13-248.65 Biotechnology |
| Zdroj: | Biotechnology Reports Biotechnology Reports, Vol 32, Iss, Pp e00678-(2021) |
| ISSN: | 2215-017X |
| Popis: | Highlights • B-3531D is the first E. alkaliphilum strain with fully assembled genome. • It is the first E. alkaliphilum strain with the ability to utilize crude oil. • Strain utilized 34.5% of crude oil for 14 days at 28 °C and a salinity of 11%. The aim of the work was to carry out the physiological, biochemical and genetic characterization of the Exiguobacterium alkaliphilum B-3531D strain. This strain is promising for use in the field of environmental biotechnology, since it has a pronounced ability to utilize crude oil and individual hydrocarbons in a wide temperature range. The genome of the strain was sequenced and completely assembled, it consists of a 2,903,369 bp circular chromosome and two circular plasmids, namely, pE73 (73,590 bp) and pE52 (52,125 bp). When cultivated in a mineral medium containing 2% of crude oil, the strain utilized 50% within 30 days of the experiment. In simulated seawater with the same oil content, the loss of hydrocarbons was 45% over the same period. For the first time we observed in an E. alkaliphilum strain the ability to efficiently utilize crude oil, including with an increased content of sodium chloride in the cultivation medium. |
| Databáze: | OpenAIRE |
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