Distinct peripheral vs mucosal T-cell phenotypes in chlamydia-infected women
| Autor: | Rakesh K. Bakshi, Richa Kapil, Ladraka' T. Brown, Brian M. O. Ogendi, William M. Geisler, Jeannette Y. Lee, Steffanie Sabbaj |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Adult T cell Immunology CCR4 C-C chemokine receptor type 7 Cell Separation Biology CD38 Adaptive Immunity CXCR3 Lymphocyte Activation Peripheral blood mononuclear cell Article Immunophenotyping 03 medical and health sciences Chemokine receptor Mice 0302 clinical medicine T-Lymphocyte Subsets medicine Immunology and Allergy Animals Humans Chlamydia Blood Cells Mucous Membrane Obstetrics and Gynecology Chlamydia Infections Th1 Cells Acquired immune system Flow Cytometry 030104 developmental biology medicine.anatomical_structure Phenotype Reproductive Medicine Vagina Female 030215 immunology |
| Zdroj: | American journal of reproductive immunology (New York, N.Y. : 1989). 78(6) |
| ISSN: | 1600-0897 |
| Popis: | Problem Differences in circulating (peripheral) and mucosal T-cell phenotypes in chlamydia-infected women remain largely unknown. Method of study Thirteen paired mononuclear cell specimens from blood and cervicovaginal lavages collected from chlamydia-infected women were stained and analyzed using ten-color cell surface flow cytometry for T-cell distribution, activation status, homing, and T helper (Th)-associated chemokine receptors (CKRs). Results A higher proportion of genital mucosal T-cells were activated (CD38+ HLA-DR+ ) and expressed CCR5 and Th1-associated CKR CXCR3+ CCR5+ compared to peripheral T-cells, but a lower proportion of mucosal T-cells expressed homing CKR CCR7, Th-2 associated CKR CCR4, and CXCR3+ CCR4+ for both T-cell subsets. Conclusion T-cell phenotypes differed in the peripheral vs genital mucosa compartments in chlamydia-infected women. As chlamydia infects mucosal epithelial cells, the finding of a higher frequency of activated T-cells and Th-1 phenotypes in the mucosa likely reflects an adaptive immune response to infection. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |