Protocols for the preparation and characterization of decellularized tissue and organ scaffolds for tissue engineering
| Autor: | Dar-Jen Hsieh, Hung-Chou Wang, Yih-Wen Tarng, Yi Chun Yeh, Periasamy Srinivasan, Ko-Chung Yen, Yun-Ju Chen |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Swine
Economic shortage Biocompatible Materials 02 engineering and technology Regenerative Medicine Regenerative medicine General Biochemistry Genetics and Molecular Biology law.invention Extracellular matrix 03 medical and health sciences Tissue engineering law Animals Humans Organ regeneration 030304 developmental biology 0303 health sciences 3D bioprinting Decellularization Tissue Engineering Tissue Scaffolds Chemistry 021001 nanoscience & nanotechnology Extracellular Matrix 0210 nano-technology Biotechnology Biomedical engineering |
| Zdroj: | BioTechniques. 70(2) |
| ISSN: | 1940-9818 |
| Popis: | Extracellular matrix (ECM) scaffolds are extensively used in tissue engineering studies and numerous clinical applications for tissue and organ reconstructions. Due to the global severe shortage of human tissues and organs, xenogeneic biomaterials are a common source for human tissue engineering and regenerative medicine applications. Traditional methods for decellularization often disrupt the 3D architecture and damage the structural integrity of the ECM scaffold. To efficiently obtain natural ECM scaffolds from animal tissues and organs with intact architecture, we have developed a platform decellularization process using supercritical CO2 and tested its potential application in tissue engineering. A combination of human mesenchymal stem cells with a decellularized dermal matrix scaffold allowed complete regeneration of skin structure in a porcine full-thickness wound model. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|