Genome-wide MyoD Binding in Skeletal Muscle Cells: A Potential for Broad Cellular Reprogramming
| Autor: | Deepayan Sarkar, Jerry Davison, Martin Morgan, Maura H. Parker, Gilson J. Sanchez, Michael S. Lawrence, Stephen J. Tapscott, Kyle L. MacQuarrie, Walter L. Ruzzo, Zizhen Yao, Yi Cao, Robert Gentleman |
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| Rok vydání: | 2010 |
| Předmět: |
animal structures
PROTEINS Cellular differentiation Amino Acid Motifs Muscle Fibers Skeletal DEVBIO Biology MyoD Models Biological General Biochemistry Genetics and Molecular Biology Cell Line Histones Mice 03 medical and health sciences 0302 clinical medicine MyoD Protein Animals Myocyte Muscle Skeletal Molecular Biology Myogenin Oligonucleotide Array Sequence Analysis 030304 developmental biology 0303 health sciences Binding Sites Genome PITX2 Myogenesis Cell Differentiation Cell Biology DNA Fibroblasts musculoskeletal system Molecular biology Gene Expression Regulation Chromatin immunoprecipitation tissues 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Developmental Cell. 18(4):662-674 |
| ISSN: | 1534-5807 |
| DOI: | 10.1016/j.devcel.2010.02.014 |
| Popis: | SummaryRecent studies have demonstrated that MyoD initiates a feed-forward regulation of skeletal muscle gene expression, predicting that MyoD binds directly to many genes expressed during differentiation. We have used chromatin immunoprecipitation and high-throughput sequencing to identify genome-wide binding of MyoD in several skeletal muscle cell types. As anticipated, MyoD preferentially binds to a VCASCTG sequence that resembles the in vitro-selected site for a MyoD:E-protein heterodimer, and MyoD binding increases during differentiation at many of the regulatory regions of genes expressed in skeletal muscle. Unanticipated findings were that MyoD was constitutively bound to thousands of additional sites in both myoblasts and myotubes, and that the genome-wide binding of MyoD was associated with regional histone acetylation. Therefore, in addition to regulating muscle gene expression, MyoD binds genome wide and has the ability to broadly alter the epigenome in myoblasts and myotubes. |
| Databáze: | OpenAIRE |
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