Highly Selective Capture Surfaces on Medical Wires for Fishing Tumor Cells in Whole Blood
| Autor: | Klaus Lücke, Jürgen Rühe, Frank D. Scherag, Solveigh Krusekopf, Thomas Brandstetter, Robert Niestroj-Pahl |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cell Survival Surface Properties Nanotechnology Tumor cells Breast Neoplasms Enzyme-Linked Immunosorbent Assay 02 engineering and technology Antibodies Analytical Chemistry 03 medical and health sciences chemistry.chemical_compound Circulating tumor cell Coated Materials Biocompatible Cell Line Tumor Neoplasms Nucleic Acids medicine Humans Whole blood Leukemia biology Cancer Reproducibility of Results Epithelial cell adhesion molecule Microfluidic Analytical Techniques 021001 nanoscience & nanotechnology Highly selective medicine.disease Epithelial Cell Adhesion Molecule Neoplastic Cells Circulating In vitro Cell biology 030104 developmental biology chemistry biology.protein Female Antibody 0210 nano-technology Antibodies Immobilized |
| Zdroj: | Analytical chemistry. 89(3) |
| ISSN: | 1520-6882 |
| Popis: | The detection of circulating tumor cells (CTCs) in the blood of cancer patients is a challenging task. CTCs are, especially at the early stages of cancer development, extremely rare cells hidden in a vast background of regular blood cells. We describe a new strategy for the isolation of CTCs from whole blood. The key component is a medical wire coated with a multilayer assembly that allows highly specific capture of EpCAM (epithelial cell adhesion molecule) positive CTCs from blood. The assembly is generated in a layer-by-layer fashion through photochemically induced C,H insertion reactions and consists of a protective layer, which shields the contacting solution from the metal, a protein resistant layer, which prevents nonspecific interactions with proteins and a layer containing the EpCAM antibodies. In vitro experiments show that these surfaces can capture tumor cells from whole blood with enrichment factors (specifically vs nonspecifically bound cells) of up to about 3000 compared to the number of leucocytes in the blood. The purity of the isolated cells is greater than 90%. After "fishing" them from the blood, the cells, still bound to the wire, can be genetically analyzed. This demonstrates that this strategy might prove useful for next generation sequencing. |
| Databáze: | OpenAIRE |
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