Structural characterization of sulfated arabinans extracted fromCladophora glomerataKützing and their macrophage activation
| Autor: | Utoomporn Surayot, Chartchai Kanongnuch, Yuwadee Peerapornpisal, SangGuan You, WooJung Park, Ju Hun Lee |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Arabinose Rhamnose Size-exclusion chromatography Fraction (chemistry) Xylose Biology Nitric Oxide Polysaccharide 01 natural sciences Applied Microbiology and Biotechnology Biochemistry Cell Line Analytical Chemistry Mice 03 medical and health sciences chemistry.chemical_compound Sulfation Adjuvants Immunologic Chlorophyta Polysaccharides Animals Molecular Biology chemistry.chemical_classification Chromatography Plant Extracts Sulfates 010405 organic chemistry Macrophages Organic Chemistry Galactose Water General Medicine Macrophage Activation 0104 chemical sciences Molecular Weight Glucose 030104 developmental biology Carbohydrate Sequence Solubility chemistry Cytokines Biotechnology |
| Zdroj: | Bioscience, Biotechnology, and Biochemistry. 80:972-982 |
| ISSN: | 1347-6947 0916-8451 |
| DOI: | 10.1080/09168451.2015.1132149 |
| Popis: | Water-soluble sulfated heteropolysaccharides were extracted from Cladophora glomerata Kützing and fractionated by ion-exchange chromatography, which yielded two subfractions, F1 and F2. The crude and fractionated polysaccharides (F1 and F2) mostly consisted of carbohydrates (62.8–74.5%) with various amounts of proteins (9.00–17.3%) and sulfates (16.5–23.5%), including different levels of arabinose (41.7–54.4%), galactose (13.5–39.0%), glucose (0.80–10.6%), xylose (6.84–13.4%), and rhamnose (0.20–2.83%). Based on the size exclusion chromatography (SEC) profiles, the crude and fractions mainly contained one peak with shoulders having molecular weight (Mw) ranges of 358–1,501 × 103. The F1 fraction stimulated RAW264.7 cells to produce considerable amounts of nitric oxide and cytokines compared to the crude and F2 fraction. The backbone of the most potent immunostimulating fraction (F1) was α-(1→4)-L-arabinopyranoside with galactose and xylose residues as branches at O-2 position, and sulfates mainly at O-2 position as well. |
| Databáze: | OpenAIRE |
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