Simultaneous profiling of 17 steroid hormones for the evaluation of endocrine-disrupting chemicals in H295R cells
| Autor: | Eiichiro Fukusaki, Kazuya Ishida, Toshiyuki Yamashita, Udi Jumhawan, Takeshi Bamba |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty medicine.medical_treatment Clinical Biochemistry Cell Endocrine Disruptors 010501 environmental sciences Pharmacology 01 natural sciences Gas Chromatography-Mass Spectrometry Cell Line Analytical Chemistry Steroid 03 medical and health sciences Tandem Mass Spectrometry Internal medicine medicine Humans Endocrine system General Pharmacology Toxicology and Pharmaceutics 0105 earth and related environmental sciences Chemistry Screening assay General Medicine Hormones Medical Laboratory Technology 030104 developmental biology medicine.anatomical_structure Endocrinology Metabolome Steroids Hormone |
| Zdroj: | Bioanalysis. 9:67-69 |
| ISSN: | 1757-6199 1757-6180 |
| Popis: | Aim: There is urgent need to develop a new protocol for the evaluation of chemical substances to potentially interact with the endocrine system and induce numerous pathological issues. The recently validated in vitro screening assay is limited on monitoring two steroid hormones. Methodology & results: The H295R model cell was exposed to seven endocrine disrupting chemicals (EDCs). The levels of 17 steroid hormones in cell extracts were subsequently determined by a quantitative targeted GC/MS/MS method. Through wide coverage, this system managed to capture the effects of exposure to increasing EDCs concentrations in the entire steroidogenic pathways. Conclusion: The developed approach could be beneficial for the mechanistic investigation of EDCs. |
| Databáze: | OpenAIRE |
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