DNA isolation from achieved formalin-fixed paraffin-embedded tissues in a series of 212 thyroid carcinoma cases: the influence of preanalytical factors on DNA quantity and purity
| Autor: | Adela Nechifor-Boilă, Claudia Bănescu, Emoke Szasz, Angela Borda, Valeriu Moldovan, Ancuta Elena Zahan |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Formalin fixed paraffin embedded Routine practice Real-Time Polymerase Chain Reaction General Biochemistry Genetics and Molecular Biology Statistics Nonparametric Specimen Handling Thyroid carcinoma 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Fluorometer Formaldehyde Carcinoma medicine Humans Thyroid Neoplasms Paraffin Embedding Chemistry Dna concentration General Medicine DNA medicine.disease DNA extraction Molecular biology Tumor Burden 030104 developmental biology 030220 oncology & carcinogenesis |
| Zdroj: | Journal of investigative medicine : the official publication of the American Federation for Clinical Research. 68(3) |
| ISSN: | 1708-8267 |
| Popis: | Objective The present study aimed to investigate the influence of several important preanalytical factors (storage period of the tumor block, maximal diameter of the tumor circled area, tumor volume and tumor fraction) on the isolated DNA from formalin-fixed paraffin-embedded (FFPE) tissues in a series of thyroid carcinomas. Design Our study included 212 FFPE blocks, archived in the Department of Pathology, Târgu-Mureş Emergency County Hospital for up to 10 years. DNA isolation was performed using a commercially available kit (MasterPure DNA purification kit, Epicentre). The DNA parameters (concentration and purity) were determined using a spectrophotometer and the Qubit 2.0 Fluorometer (Thermo Fisher Scientific) for an accurate and sensitive DNA quantification. Results The mean DNA concentration and purity for the study cases were 489.3±372.6 ng/µl and 1.667±0.1912, respectively. The DNA concentration was correlated with the maximal diameter of the tumor circled area (pConclusion The maximal diameter of the tumor circled area, tumor volume and tumor fraction are important morphological factors that correlate with the DNA concentration and should be carefully assessed in routine practice prior to performing DNA isolation from FFPE tissues. |
| Databáze: | OpenAIRE |
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