Resveratrol depresses the growth of colorectal aberrant crypt foci by affecting bax and p21(CIP) expression
| Autor: | Ivana Sarotto, Giovanna Caderni, Annalisa Davit, Luciana Tessitore |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Cyclin-Dependent Kinase Inhibitor p21
Male medicine.medical_specialty Cancer Research Colon Azoxymethane Resveratrol Biology medicine.disease_cause digestive system chemistry.chemical_compound Bcl-2-associated X protein Intestinal mucosa Internal medicine Cyclins Proto-Oncogene Proteins Stilbenes medicine Animals Anticarcinogenic Agents Intestinal Mucosa Anticarcinogen bcl-2-Associated X Protein Rectum General Medicine Antineoplastic Agents Phytogenic digestive system diseases Growth Inhibitors Rats Inbred F344 Rats Endocrinology chemistry Proto-Oncogene Proteins c-bcl-2 Apoptosis biology.protein Carcinogens Carcinogenesis Colorectal Neoplasms Precancerous Conditions Cell Division Aberrant crypt foci |
| Zdroj: | Carcinogenesis. 21(8) |
| ISSN: | 0143-3334 |
| Popis: | We investigated whether resveratrol (RV) affects azoxymethane (AOM)-induced colon carcinogenesis, by administering RV (200 microg/kg/day in drinking water) to male F344 rats for 100 days, beginning 10 days before carcinogen treatment (two weekly doses of 15 mg/kg AOM). Aberrant crypt foci (ACF) were isolated and proliferation, apoptosis and expression of the cell cycle genes bax and p21 were determined. RV significantly reduced the number of ACF/colon [25.7 +/- 3.6 (mean +/- SEM) versus 39.4 +/- 3.3 in controls; P < 0.01] and their multiplicity (2.7 +/- 0.3 versus 4.9 +/- 0.6 in controls; P < 0.01), and also abolished large ACF. In RV-treated rats, bax expression was enhanced in ACF but not in the surrounding mucosa. In both controls and RV-treated rats, proliferation was higher in ACF than in normal mucosa. p21 was expressed in ACF of controls and of RV-treated rats and in normal mucosa of controls, but was lost in normal mucosa of RV-treated animals. In conclusion, the results suggest a protective role of RV in colon carcinogenesis with a mechanism involving changes in bax and p21 expression. |
| Databáze: | OpenAIRE |
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