Effect of apoA-I on cholesterol release and apoE secretion in human mature adipocytes
| Autor: | Evelyne Tarnus, Christophe Gardes, Roger G. Clerc, Laurence Hoareau, Régis Roche, Maya Cesari, Ravi Kumar Murumalla, Karima Bencharif, Frank Tallet |
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| Přispěvatelé: | Groupe d'Etude sur l'Inflammation Chronique et l'Obésité (GEICO), Université de La Réunion (UR), Groupe d'Etude sur l'Inflammation Chronique et l'Obésité ( GEICO ), Université de la Réunion ( UR ) |
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Apolipoprotein E
Time Factors Apolipoprotein B Endocrinology Diabetes and Metabolism Clinical Biochemistry Adipose tissue Second Messenger Systems chemistry.chemical_compound 0302 clinical medicine Endocrinology Adipocytes Cyclic AMP Enzyme Inhibitors lcsh:RC620-627 Cells Cultured ComputingMilieux_MISCELLANEOUS 0303 health sciences Secretory Pathway Reverse cholesterol transport Middle Aged lcsh:Nutritional diseases. Deficiency diseases Cholesterol Cardiovascular Diseases 030220 oncology & carcinogenesis Female lipids (amino acids peptides and proteins) ATP Binding Cassette Transporter 1 Adult medicine.medical_specialty Subcutaneous Fat Medical Biochemistry Biology Apolipoproteins E 03 medical and health sciences [ SDV.MHEP ] Life Sciences [q-bio]/Human health and pathology Internal medicine medicine Humans Secretion 030304 developmental biology Lipidology Biochemistry medical Apolipoprotein A-I Research Biochemistry (medical) nutritional and metabolic diseases Atherosclerosis Gene Expression Regulation chemistry ABCA1 biology.protein ATP-Binding Cassette Transporters Hydroxymethylglutaryl CoA Reductases [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology |
| Zdroj: | Lipids in Health and Disease, Vol 9, Iss 1, p 75 (2010) Lipids in Health and Disease Lipids in Health and Disease, BioMed Central, 2010, 9 (1), pp.1--9. ⟨10.1186/1476-511X-9-75⟩ Lipids in Health and Disease, BioMed Central, 2010, 9 (1), pp.1--9. 〈10.1186/1476-511X-9-75〉 |
| ISSN: | 1476-511X |
| Popis: | Background The risk of cardiovascular disease is inversely correlated to level of plasma HDL-c. Moreover, reverse cholesterol transport (RCT) from peripheral tissues to the liver is the most widely accepted mechanism linked to the anti-atherosclerotic activity of HDL. The apolipoprotein A-I (apoA-I) and the ABC transporters play a key role in this process. Adipose tissue constitutes the body's largest pool of free cholesterol. The adipose cell could therefore be regarded as a key factor in cholesterol homeostasis. The present study investigates the capacity of primary cultures of mature human adipocytes to release cholesterol and explores the relationships between apoA-I, ABCA1, and apoE as well as the signaling pathways that could be potentially involved. Results We demonstrate that apoA-I induces a strong increase in cholesterol release and apoE secretion from adipocytes, whereas it has no transcriptional effect on ABCA1 or apoE genes. Furthermore, brefeldin A (BFA), an intracellular trafficking inhibitor, reduces basal cholesterol and apoE secretion, but does not modify induction by apoA-I. The use of statins also demonstrates that apoA-I stimulated cholesterol release is independent of HMG-CoA reductase activation. Conclusion Our work highlights the fact that adipose tissue, and particularly adipocytes, may largely contribute to RCT via a mechanism specifically regulated within these cells. This further supports the argument that adipose tissue must be regarded as a major factor in the development of cardiovascular diseases, in particular atherosclerosis. |
| Databáze: | OpenAIRE |
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