Structure and expression of an ovine endometrial oxytocin receptor cDNA
| Autor: | D R E Abayasekara, Anthony P.F. Flint, Paul R. Riley, H J Stewart |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
DNA
Complementary Swine G protein Molecular Sequence Data Gene Expression Transfection Polymerase Chain Reaction Cell Line Endometrium Endocrinology Estrus Species Specificity In vivo Complementary DNA Chlorocebus aethiops Animals Humans Amino Acid Sequence RNA Messenger Receptor Molecular Biology DNA Primers Gene Library chemistry.chemical_classification Messenger RNA Sheep Base Sequence Sequence Homology Amino Acid Oxytocin receptor Molecular biology Recombinant Proteins Rats Amino acid chemistry Receptors Oxytocin Female Signal transduction Oligonucleotide Probes |
| Zdroj: | Journal of Molecular Endocrinology. 15:195-202 |
| ISSN: | 1479-6813 0952-5041 |
| DOI: | 10.1677/jme.0.0150195 |
| Popis: | A sheep endometrial oxytocin receptor (OTR) cDNA (1·5 kb) was isolated from a λ-ZAP library using a reverse transcription-PCR product probe generated from oestrous endometrial mRNA. The sheep OTR cDNA shared an overall similarity of 82% with human OTR cDNA, 85% with pig OTR cDNA and 76% with rat OTR cDNA. The encoded receptor was a 391 amino acid polypeptide 94% similar to human OTR, 94% similar to pig OTR and 93% similar to rat OTR. The sheep OTR contained two additional amino acids compared with human OTR which were located in the highly GC-rich third intracytoplasmic loop. This region is thought to be associated with G protein coupling and signal transduction. Expression of the cDNA in Cos-7 cells and measurement of oxytocin-induced phosphoinositide turnover confirmed that it coded for a functional product. The affinity of the expressed receptor was comparable with that observed for the in vivo receptor. |
| Databáze: | OpenAIRE |
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