Simple multichannel system for the measurement of the net water flux across biological tissues
Autor: | Ricardo Dorr, Luis E. Fernández, Francisco A. Uzal, Mariano E. Fernandez-Miyakawa, Cristina Ibarra |
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Rok vydání: | 2007 |
Předmět: |
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Membranes Materials science Water transport SIMPLE (military communications protocol) Clostridium perfringens business.industry Acoustics Flux Epithelial Cells Health Informatics Computing Methodologies Computer Science Applications Membrane Software Body Water Humans Meniscus Tube (fluid conveyance) business |
Zdroj: | Computer Methods and Programs in Biomedicine. 85:95-100 |
ISSN: | 0169-2607 |
DOI: | 10.1016/j.cmpb.2006.09.016 |
Popis: | This paper describes the development of a simple system for measurement of net water movement through biological membrane barriers. The system is based on the detection of a water meniscus inside a polyethylene tube, which reflects the water movement inside one hemichamber of a modified Ussing chamber containing a membrane barrier. The detection device consists of a commercial computer-controlled flat bed scanner and specifically developed software. This system allows one to perform a relatively high number of individual experiments per physical unit. It is a flexible and affordable device, which allows comparatively more information per unit to be obtained than previously described methods. |
Databáze: | OpenAIRE |
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