Simple multichannel system for the measurement of the net water flux across biological tissues

Autor: Ricardo Dorr, Luis E. Fernández, Francisco A. Uzal, Mariano E. Fernandez-Miyakawa, Cristina Ibarra
Rok vydání: 2007
Předmět:
Zdroj: Computer Methods and Programs in Biomedicine. 85:95-100
ISSN: 0169-2607
DOI: 10.1016/j.cmpb.2006.09.016
Popis: This paper describes the development of a simple system for measurement of net water movement through biological membrane barriers. The system is based on the detection of a water meniscus inside a polyethylene tube, which reflects the water movement inside one hemichamber of a modified Ussing chamber containing a membrane barrier. The detection device consists of a commercial computer-controlled flat bed scanner and specifically developed software. This system allows one to perform a relatively high number of individual experiments per physical unit. It is a flexible and affordable device, which allows comparatively more information per unit to be obtained than previously described methods.
Databáze: OpenAIRE