Morphometric and Nanomechanical Features of Platelets from Women with Early Pregnancy Loss Provide New Evidence of the Impact of Inherited Thrombophilia

Autor: Tonya D. Andreeva, Ariana Langari, Stefka G. Taneva, Andrey Tchorbanov, Svetla Todinova, Ina Giosheva, Nikolina Mihaylova, Regina Komsa-Penkova, Galya B. Georgieva, Georgi M. Golemanov, Sashka Krumova
Rok vydání: 2021
Předmět:
0301 basic medicine
polymorphisms in thrombophilia genes
Polymorphisms in thrombophilia genes
030204 cardiovascular system & hematology
Miscarriage
Atomic force microscopy
0302 clinical medicine
Pregnancy
Thrombophilia
Platelet
Flow cytometry
Biology (General)
Spectroscopy
atomic force microscopy
medicine.diagnostic_test
Chemistry
General Medicine
Computer Science Applications
platelets
Female
Adult
Blood Platelets
Platelets
Abortion
Habitual
medicine.medical_specialty
QH301-705.5
Early Pregnancy Loss
Early pregnancy loss
Article
Catalysis
Inorganic Chemistry
03 medical and health sciences
Internal medicine
medicine
Humans
Physical and Theoretical Chemistry
Inherited thrombophilia
QD1-999
Molecular Biology
Polymorphism
Genetic
flow cytometry
Organic Chemistry
early pregnancy loss
medicine.disease
Nanostructures
030104 developmental biology
Endocrinology
Case-Control Studies
Zdroj: International Journal of Molecular Sciences, Vol 22, Iss 7778, p 7778 (2021)
International Journal of Molecular Sciences
Volume 22
Issue 15
ISSN: 1422-0067
Popis: Pregnancy is associated with hypercoagulation states and increased thrombotic risk, especially in women with thrombophilia. We combine atomic force microscopy (AFM) and flow cytometry to examine the morphology and nanomechanics of platelets derived from women with early pregnancy loss (EPL) and control pregnant (CP) and non-pregnant (CNP) women. Both control groups exhibit similar morphometric parameters (height and surface roughness) and membrane stiffness of platelets. EPL patients’ platelets, on the other hand, are more activated than the control groups, with prominent cytoskeletal rearrangement. In particular, reduced membrane roughness (22.9 ± 6 nm vs. 39.1 ± 8 nm) (p <
0.05) and height (692 ± 128 nm vs. 1090 ± 131 nm) (p <
0.05), strong alteration in the membrane Young modulus, increased production of platelets’ microparticles, and higher expression of procoagulant surface markers, as well as increased occurrence of thrombophilia (FVL, FII20210A, PLA1/A2, MTHFR 
C677T or 4G/5G PAI-1) polymorphisms were found. We suggest that the carriage of thrombophilic mutations triggers structural and nanomechanical abnormalities in platelets, resulting in their increased activation. The activation state of platelets can be well characterized by AFM, and the morphometric and nanomechanical characteristics might serve as a new criterion for evaluation of the cause of miscarriage and offer the prospect of an innovative approach serving for diagnostic purposes.
Databáze: OpenAIRE
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