First molecular identification and report of genetic diversity of Strongyloides stercoralis, a current major soil-transmitted helminth in humans from Lao People's Democratic Republic
| Autor: | Sakhone Laymanivong, Lakkhana Sadaow, Wanchai Maleewong, Jurairat Jongthawin, Bounnaloth Insisiengmay, Pinnakhone Laxachack, Pewpan M. Intapan, Bouasy Hangvanthong, Issarapong Phosuk, Oranuch Sanpool, Viengxay Vanisaveth, Rutchanee Rodpai, Tongjit Thanchomnang |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Adult Male Veterinary medicine Adolescent 030231 tropical medicine Helminthiasis Strongyloides stercoralis 03 medical and health sciences Feces Soil Young Adult 0302 clinical medicine Helminths parasitic diseases medicine Prevalence Animals Humans Child Phylogeny Aged Aged 80 and over General Veterinary biology Coinfection Genetic Variation General Medicine 030108 mycology & parasitology Middle Aged medicine.disease biology.organism_classification Infectious Diseases Strongyloidiasis Cross-Sectional Studies Opisthorchiasis Laos Insect Science Child Preschool Schistosoma mekongi Trichuris trichiura Parasitology Female Ascaris lumbricoides |
| Zdroj: | Parasitology research. 115(8) |
| ISSN: | 1432-1955 |
| Popis: | Strongyloidiasis is a major soil-transmitted helminth (STH) disease that affects people worldwide. We present updated data on prevalence in the Lao People's Democratic Republic (Lao PDR) in 2015, arising from a community cross-sectional helminthiasis survey. Fecal samples were collected from 327 individuals across three provinces in Lao PDR (Luang Prabang in the north, Khammouane in the center, and Champasack in the south). Agar plate culture and Kato-Katz methods were used to examine duplicate stool samples from each participant to detect Strongyloides stercoralis and co-infecting helminths. Overall prevalences of S. strercoralis human hookworm, Taenia spp., Trichuris trichiura, Ascaris lumbricoides, and Enterobius vermicularis were 41.0, 28.1, 4.9, 4.0, 1.5, and 0.9 %, respectively. The prevalence of miscellaneous trematodiases (including opisthorchiasis) was 37.9 % and of Schistosoma mekongi infection was 0.3 %. Strongyloidiasis is a current major STH disease in Lao PDR. We also report the molecular-phylogenetic identification of S. stercoralis adult males collected from 40 representative human strongyliodiasis fecal samples. DNA was extracted, amplified, and sequenced from a portion of the mitochondrial cox1 gene and the nuclear 18S ribosomal DNA. Phylogenetic analyses indicated that all specimens sequenced belonged to S. stercoralis (Bavay, 1876) Stiles and Hassall, 1902. The cox1 sequences exhibited great diversity (24 haplotypes) in Lao PDR. This is the first molecular identification and report of genetic diversity of S. stercoralis in humans from Lao PDR. An effective parasite control program is needed to reduce the serious health impacts. |
| Databáze: | OpenAIRE |
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