Immunohistochemical and Ultrastructural Studies of Mycoplasma hyopneumoniae Strain in Naturally Infected Pigs in Nigeria
| Autor: | T. Nagy, O. L. Ajayi, O. O. Alaka, C. C. Brown, M. B. Ard, O. A. Mustapha, M. O. Olaniyi, J. P. Shields |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0303 health sciences
Strain (chemistry) 040301 veterinary sciences Veterinary medicine pigs 04 agricultural and veterinary sciences General Medicine Biology respiratory system biology.organism_classification Microbiology respiratory tract diseases nigeria 0403 veterinary science 03 medical and health sciences Mycoplasma hyopneumoniae immunohistochemistry SF600-1100 Ultrastructure Immunohistochemistry pathogenicity pneumonia mycoplasma hyopneumoniae ultrastructural changes 030304 developmental biology |
| Zdroj: | Folia Veterinaria, Vol 64, Iss 1, Pp 1-10 (2020) |
| ISSN: | 2453-7837 |
| Popis: | Enzootic pneumonia caused by hyopneumoniae (MHYO) remains a serious concern to the swine industry in many countries including Nigeria. MHYO strains isolated from pigs from different countries and geographical locations are known to vary in pathogenicity. There is a paucity of information on the pathogenicity of the MHYO strain affecting pigs in Nigeria. This study investigated the pathogenicity of the MHYO strain in naturally infected pigs using immunohisto-chemistry and electron microscopy. Two hundred and sixty four lungs of slaughtered pigs were randomly collected from abattoirs at Abeokuta, Ibadan and Lagos, in Southwest Nigeria. A sub-sample of 104 pneumonic and 20 apparently normal lungs was selected, processed for routine histopathological examination and immunohistochemistry, while 3 lung tissues samples were selected for ultrastructural studies. The most significant microscopic changes observed were suppurative broncho-interstitial pneumonia associated with varying degrees of lymphoid hyperplasia of the bronchus-associated lymphoid tissue (BALT) and thickened alveolar septa due to cellular infiltration consisting predominantly of neutrophils and a few mononuclear cells. Immunohistochemically, MHYO antigen was detected in 86/104 (82.69 %) of MHYO-infected lung tissues and typically exhibited a granular brown reaction on the bronchial and bronchiolar epithelial lining, mononuclear cells in the BALT and luminal cellular exudates within the airways. Transmission electron microscopy revealed numerous Mycoplasma organisms in the lumina of the airways, in between degenerated cilia, while a few Mycoplasmas were located within the alveoli. It was concluded that the MHYO strain detected in this study was pathogenic to pigs and capable of inducing pneumonia, and therefore implicated in the pathogenesis. |
| Databáze: | OpenAIRE |
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