Zoledronic acid inhibits human nasopharyngeal carcinoma cell proliferation by activating mitochondrial apoptotic pathway
Autor: | Ying-cheng Lin, Wen-zhao Lin, Wen Lin, Sui-ling Lin, Wan-lan Huang, Xuyuan Li, Hong-biao Wang |
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Rok vydání: | 2012 |
Předmět: |
Cancer Research
Cell Blotting Western Gene Expression Antineoplastic Agents Apoptosis Biology Real-Time Polymerase Chain Reaction Zoledronic Acid Flow cytometry chemistry.chemical_compound Cell Line Tumor medicine In Situ Nick-End Labeling Humans MTT assay Propidium iodide Cell Proliferation TUNEL assay Nasopharyngeal Carcinoma medicine.diagnostic_test Diphosphonates Cell growth Reverse Transcriptase Polymerase Chain Reaction Carcinoma Imidazoles Nasopharyngeal Neoplasms Hematology General Medicine Cell cycle Flow Cytometry Molecular biology Mitochondria medicine.anatomical_structure Oncology chemistry |
Zdroj: | Medical oncology (Northwood, London, England). 29(5) |
ISSN: | 1559-131X |
Popis: | We investigated the apoptosis-inducing effect of zoledronic acid in human nasopharyngeal carcinoma cell HNE-1 and explore the potential mechanism. Human nasopharyngeal carcinoma cell HNE-1 was exposed to various concentrations (0–40 μmol/L) of zoledronic acid. Cell proliferation was studied by an MTT assay. Cell apoptosis was analyzed by flow cytometry and TdT-mediated dUTP nick-end labeling (TUNEL) assay. Cell cycle was analyzed by flow cytometry. Gene expressions were investigated by quantitative real-time PCR, and protein expressions were investigated by Western blot. The results showed zoledronic acid decreased cell proliferation not in a time- or dose-dependent fashion. TUNEL assay, together with Annexin V/propidium iodide FACS analysis, confirmed the increase in apoptotic HNE-1 cells treated with zoledronic acid. Cell cycle analysis showed a larger number of treated cells occupied the S-phase. Quantitative RT-PCR and Western blot revealed that the pro-apoptotic genes, Bad, Bax, and Caspase-9, were upregulated in treated HNE-1 cells, whereas the anti-apoptotic gene, Bcl-2, was downregulated in both mRNA and protein levels. In conclusion, zoledronic inhibits human nasopharyngeal carcinoma cell proliferation by inducing apoptosis via the mitochondrial apoptotic pathway. |
Databáze: | OpenAIRE |
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