Reverse Genetics and High Throughput Sequencing Methodologies for Plant Functional Genomics
| Autor: | Götz M. Reustle, Anis Ben-Amar, Ahmed Mliki, Samia Daldoul, Gabriele Krczal |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Computer science Systems biology Functional genomics Computational biology Gene Annotation Bioinformatics Omics platforms DNA sequencing Reverse genetics Article 03 medical and health sciences 030104 developmental biology Genetics Large-scale sequencing Large-Scale Sequencing Gene Genetics (clinical) Organism |
| Zdroj: | Current Genomics |
| ISSN: | 1875-5488 1389-2029 |
| Popis: | In the post-genomic era, increasingly sophisticated genetic tools are being developed with the long-term goal of understanding how the coordinated activity of genes gives rise to a complex organism. With the advent of the next generation sequencing associated with effective computational approaches, wide variety of plant species have been fully sequenced giving a wealth of data sequence information on structure and organization of plant genomes. Since thousands of gene sequences are already known, recently developed functional genomics approaches provide powerful tools to analyze plant gene functions through various gene manipulation technologies. Integration of different omics platforms along with gene annotation and computational analysis may elucidate a complete view in a system biology level. Extensive investigations on reverse genetics methodologies were deployed for assigning biological function to a specific gene or gene product. We provide here an updated overview of these high throughout strategies highlighting recent advances in the knowledge of functional genomics in plants. |
| Databáze: | OpenAIRE |
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