Self-Assembled Oligo-Urethane Nanoparticles: Their Characterization and Use for the Delivery of Active Biomolecules into Mammalian Cells

Autor:	Suja Shrestha, Meghan J. McFadden, Allen C. T. Teng, Patrick Dong Min Chang, Joyce Deng, Tatianna W. Y. Wong, Ronald D. Cohn, Evgueni A. Ivakine, Anthony O. Gramolini, J. Paul Santerre
Rok vydání:	2021
Předmět:	Molecular Structure Cell Survival Oligonucleotides Biocompatible Materials Mice Drug Delivery Systems HEK293 Cells Materials Testing Animals Humans Nanoparticles General Materials Science Luciferases Cells Cultured
Zdroj:	ACS Applied Materials & Interfaces. 13:58352-58368
ISSN:	1944-8252 1944-8244
Popis:	Developing safe and effective strategies to deliver biomolecules such as oligonucleotides and proteins into cells has grown in importance over recent years, with an increasing demand for non-viral methods that enable clinical translation. Here, we investigate uniquely configured oligo-urethane nanoparticles based on synthetic chemistries that minimize the release of pro-inflammatory biomarkers from immune cells, show low cytotoxicity in a broad range of cells, and efficiently deliver oligonucleotides and proteins into mammalian cells. The mechanism of cell uptake for the self-assembled oligo-urethane nanoparticles was shown to be directed by caveolae-dependent endocytosis in murine myoblasts (C
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::263f013586bb0dd551cd3774b30b5414 https://doi.org/10.1021/acsami.1c17868 Zobrazit plný text záznamu