Fluorescence imaging host pathogen interactions: fifteen years benefit of hindsight…

Autor: Nathalie Aulner, Audrey Salles, Pascal Roux, Spencer L. Shorte, Anne Danckaert, Julien Fernandes, Jean-Yves Tinevez, Marie-Anne Nicola
Přispěvatelé: BioImagerie Photonique – Photonic BioImaging (UTechS PBI), Institut Pasteur [Paris] (IP), We are grateful for support from the 7th Framework Program of the European Commission (LEISHDRUG project, 223414), the French Government (L’Agence nationale de la recherche (ANR)) programmes: Investissements d’Avenir programme (‘Laboratoire d’Excellence Integrative Biology of Emerging Infectious Diseases’, grant ANR-10-LABX-62-IBEID and ‘Laboratoire Revive’, grant ANR 10-LBX-73-REVIVE), France BioImaging (FBI, grant ANR-10-INSB-04-01), the Région Ile de France (Domaine d’intêret majeur Innovative technologies for life sciences, DIM ELICIT) and the GIS IBiSA (Infrastructures en biologie santé et agronomie) and the Institut Pasteur., ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), ANR-10-LABX-0073,REVIVE,Stem Cells in Regenerative Biology and Medicine(2010), European Project: 223414,EC:FP7:HEALTH,FP7-HEALTH-2007-B,LEISHDRUG(2008), Institut Pasteur [Paris]
Rok vydání: 2018
Předmět:
0301 basic medicine
Microbiology (medical)
Fluorescence-lifetime imaging microscopy
MESH: Host-Pathogen Interactions
[SDV]Life Sciences [q-bio]
ComputingMethodologies_IMAGEPROCESSINGANDCOMPUTERVISION
MESH: Automation
Laboratory
Biology
Microbiology
MESH: Containment of Biohazards
03 medical and health sciences
0302 clinical medicine
Optical imaging
MESH: Laboratories/organization & administration
MESH: Microscopy
Fluorescence/methods
Humans
Automation
Laboratory
MESH: Humans
Containment of Biohazards
MESH: Molecular Imaging/instrumentation
Data science
Molecular Imaging
030104 developmental biology
Infectious Diseases
Microscopy
Fluorescence
Host-Pathogen Interactions
MESH: Microscopy
Fluorescence/instrumentation
Laboratories
MESH: Molecular Imaging/methods
Host (network)
030217 neurology & neurosurgery
Hindsight bias
Zdroj: Current Opinion in Microbiology
Current Opinion in Microbiology, 2018, 43, pp.193-198. ⟨10.1016/j.mib.2018.03.001⟩
Current Opinion in Microbiology, Elsevier, 2018, 43, pp.193-198. ⟨10.1016/j.mib.2018.03.001⟩
ISSN: 1369-5274
Popis: International audience; We consider in review current state-of-the-art fluorescence microscopy for investigating the host-pathogen interface. Our perspective is honed from years with literally thousands of microbiologists using the variety of imaging technologies available within our dedicated BSL2/BSL3 optical imaging research service facilities at the Institut Pasteur Paris founded from scratch in 2001. During fifteen years learning from the success and failures of introducing different fluorescence imaging technologies, methods, and technical development strategies we provide here a synopsis review of our experience to date and a synthesis of how we see the future in perspective for fluorescence imaging at the host-pathogen interface.
Databáze: OpenAIRE
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