Different expression of adhesion molecules on CD34+ cells in AML and B-lineage ALL and their normal bone marrow counterparts
| Autor: | B Van Camp, Fabienne Trullemans, K. Vander Gucht, Rik Schots, Nadine Balduck, K. Jochmans, Jacques Otten, I. Van Riet, Wim Renmans, P. Lacor, M. De Waele |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Integrins
Pathology medicine.medical_specialty Myeloid Receptors Lymphocyte Homing CD34 Antigens CD34 Bone Marrow Cells Integrin alpha4beta1 Biology Immunophenotyping hemic and lymphatic diseases Acute lymphocytic leukemia medicine Humans Acute leukemia Myeloid leukemia Cell Differentiation Hematology General Medicine CD58 Antigens Flow Cytometry medicine.disease Burkitt Lymphoma Lymphocyte Function-Associated Antigen-1 Platelet Endothelial Cell Adhesion Molecule-1 Leukemia medicine.anatomical_structure Leukemia Myeloid Acute Disease Cancer research Bone marrow Stem cell Cell Adhesion Molecules |
| Zdroj: | European Journal of Haematology. 63:192-201 |
| ISSN: | 1600-0609 0902-4441 |
| DOI: | 10.1111/j.1600-0609.1999.tb01767.x |
| Popis: | The expression of adhesion molecules on CD34 + cells in acute myeloid leukemia (AML) and B-lineage acute lymphoblastic leukemia (B-lineage ALL) was compared with that on the myeloid and B-lymphoid CD34 + cells in normal bone marrow. Bone marrow aspirates of 10 patients with AML, 8 patients with B-lineage ALL and of 6 healthy volunteers were examined. The phenotype of the CD34 + cells was determined with a double immunofluorescence method and flow cytometry. CD34 + cells in AML and B-lineage ALL showed a lower expression of VLA-2 and VLA-3 and a higher expression of ICAM- 1 and LFA-3 than their normal bone marrow counterparts. AML CD34 + cells had less L-selectin but more VLA-5 on their surface membrane than normal myeloid CD34 + cells. B-lineage ALL CD34 + cells showed an overexpression of LFA-3. In individual patients deficiencies or overexpression of the β1 integrin chain, VLA-4, PECAM-I or HCAM also occurred. An abnormal adhesive capacity of the leukemic cells may influence their proliferation, their localisation and apoptosis. An aberrant expression of adhesion molecules may be used for the detection of minimal residual leukemia in these patients. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text